Volume 9, Issue 3 (2018)
JMBS 2018, 9(3): 473-482 |
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Daghaghelh R, Sabouri H, Hosseini Moghaddm H, Jorjani E, Fallahi H. Mapping Genes Controlling Morphological Traits in F3 Families Caused by Becher×Kavir Cross in Barley. JMBS 2018; 9 (3) :473-482
URL: http://biot.modares.ac.ir/article-22-13139-en.html
URL: http://biot.modares.ac.ir/article-22-13139-en.html
Mapping Genes Controlling Morphological Traits in F3 Families Caused by Becher×Kavir Cross in Barley
1- Plant Production Department, Agriculture Faculty, Gonbad Kavus University, Gonbad Kavus, Iran
2- Plant Production Department, Agriculture Faculty, Gonbad Kavus University, Gonbad Kavus, Iran, End of Basirat Boulivard, Shahid Fallahi Street, Gonbad Kavus, Iran , hos.sabouri@gmail.com
3- Biology Department, Basic Sciences Faculty, Gonbad Kavus University, Gonbad Kavus, Iran
4- Research & Education Center of Agriculture & Natural Resources in Mazandaran, Agricultural Extension & Education Research Organization, Sari, Iran
2- Plant Production Department, Agriculture Faculty, Gonbad Kavus University, Gonbad Kavus, Iran, End of Basirat Boulivard, Shahid Fallahi Street, Gonbad Kavus, Iran , hos.sabouri@gmail.com
3- Biology Department, Basic Sciences Faculty, Gonbad Kavus University, Gonbad Kavus, Iran
4- Research & Education Center of Agriculture & Natural Resources in Mazandaran, Agricultural Extension & Education Research Organization, Sari, Iran
Abstract: (5002 Views)
Aims: The important achievement of genetic analysis of Quantitative trait locus (QTLs) is to facilitate the investigation of the inheritance of simple Mendelian traits. The aim of this study was mapping genes controlling morphological traits in F3 Families caused by Becher×Kavir cross in barley.
Materials and Methods: In the present experimental research, in order to map QTLs, 103 F3 families caused by Becher×Kavir cross were cultivated in a randomized complete block design with 3 replications during 2014-2015. Number of germinated seeds, during the grain filling period, plant height, peduncle length, seed weight, and harvest index were evaluated. Linkage map was prepared, using SSR, iPBS, IRAP, and ISSR marker. QTLs were identified by QGENE 4.0 software and QTL analysis was performed by composite interval mapping.
Findings: The identified QTLs justified with load score of 2.007, 8.6% of variance of phenotype germinated seed number, score of 22.2, 9.5% variance of phenotype grain filling period, score of 2.74, 1.16% of variance of plant height, score of 2.19, 9.3% of the variance of the peduncle length, the score of 2.04, 8.7% of variance of the seed weight, and with the scores of 2.38, 2.38, and 2.16 justified 10.1, 10.1, and 9.2% of the variance of the harvest index, respectively.
Conclusion: There are one QTL on chromosome 6 and ISSR38-4 closely marker for number of germinated seeds, one QTL on chromosome 7 in iPBS2076-6-iPBS2085-1 distance of marker for during the grain filling period, one QTL on chromosome 2 in iPBS2083-3-HVBKASI distance of marker for plant height, one QTL on chromosome 6 and ISSR38-4 closely marker for peduncle length, one QTL on chromosome 3 in iPBS2075-5-ISSR38-7 distance of marker for seed weight, and 3 QTLs for harvest index, respectively.
Materials and Methods: In the present experimental research, in order to map QTLs, 103 F3 families caused by Becher×Kavir cross were cultivated in a randomized complete block design with 3 replications during 2014-2015. Number of germinated seeds, during the grain filling period, plant height, peduncle length, seed weight, and harvest index were evaluated. Linkage map was prepared, using SSR, iPBS, IRAP, and ISSR marker. QTLs were identified by QGENE 4.0 software and QTL analysis was performed by composite interval mapping.
Findings: The identified QTLs justified with load score of 2.007, 8.6% of variance of phenotype germinated seed number, score of 22.2, 9.5% variance of phenotype grain filling period, score of 2.74, 1.16% of variance of plant height, score of 2.19, 9.3% of the variance of the peduncle length, the score of 2.04, 8.7% of variance of the seed weight, and with the scores of 2.38, 2.38, and 2.16 justified 10.1, 10.1, and 9.2% of the variance of the harvest index, respectively.
Conclusion: There are one QTL on chromosome 6 and ISSR38-4 closely marker for number of germinated seeds, one QTL on chromosome 7 in iPBS2076-6-iPBS2085-1 distance of marker for during the grain filling period, one QTL on chromosome 2 in iPBS2083-3-HVBKASI distance of marker for plant height, one QTL on chromosome 6 and ISSR38-4 closely marker for peduncle length, one QTL on chromosome 3 in iPBS2075-5-ISSR38-7 distance of marker for seed weight, and 3 QTLs for harvest index, respectively.
Article Type: Research Paper |
Subject:
Agricultural Biotechnology
Received: 2016/10/16 | Accepted: 2017/02/6 | Published: 2018/09/22
Received: 2016/10/16 | Accepted: 2017/02/6 | Published: 2018/09/22
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