Volume 9, Issue 3 (2018)
JMBS 2018, 9(3): 395-402 |
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Hassani L, Safaei E, Hakimian F, Soufian S. Thermodynamic Investigation of Copper Porphyrazines and Phthalocyanine Interaction with Human Telomeric G-Quadruplex DNA. JMBS 2018; 9 (3) :395-402
URL: http://biot.modares.ac.ir/article-22-13905-en.html
URL: http://biot.modares.ac.ir/article-22-13905-en.html
1- Biological Sciences Faculty, Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan, Iran
2- Chemistry Department, College of Sciences, Shiraz University, Shiraz, Iran
3- Biology Department, Basic Sciences Faculty, Arak Branch, Payam-e-Noor University, Arak, Iran, Biology Department, Arak Branch, Payam-e-Noor University, Ring Road, Elahie, Arak, Iran. Postal Code: 3818889581 , s_soofian@pnu.ac.ir
2- Chemistry Department, College of Sciences, Shiraz University, Shiraz, Iran
3- Biology Department, Basic Sciences Faculty, Arak Branch, Payam-e-Noor University, Arak, Iran, Biology Department, Arak Branch, Payam-e-Noor University, Ring Road, Elahie, Arak, Iran. Postal Code: 3818889581 , s_soofian@pnu.ac.ir
Abstract: (4553 Views)
Aims: The G-quadruplex structural motifs of DNA are considered a novel target for drug discovery. As potential compounds, small molecules that selectively target the G-quadruplex structures may be used for therapeutic purposes. The aim of this study was the thermodynamic investigation of copper porphyrazines and phthalocyanine interaction with human telomeric G-quadruplex DNA.
Materials and Methods: In the present experimental study, interaction of an anionic water-soluble phthalocyanine Cu(PcTs) and two cationic water-soluble tetrapyridinoporphyrazines, including [Cu(2,3-tmtppa)] 4+ and [Cu(3,4-tmtppa)]4+ complexes with human telomeric G-quadruplexDNA was thermodynamically investigated in different concentrations of Na+ and K+ cations, using fluorescence spectroscopy. The data were analyzed via the Stern-Volmer plot and the van't Hoff plot.
Findings: Fluorescent intercalator displacement indicated the displacement ability of the complexes with thiazole orange. Stern-Volmer plots of the porphyrazines exhibited a slight positive deviation from a straight line, suggesting both static and dynamic quenching. In addition, the quenching effect of the two porphyrazines was noticeably higher than the phthalocyanine, implying binding of Cu(PcTs) to both forms of the quadruplex was weaker compared to Cu(2,3-tmtppa) and Cu(3,4-tmtppa). Gibbs free energy (∆G) for binding was negative, implying that the interaction between the complexes and the G-quadruplex DNA was favorable thermodynamically.
Conclusion: The binding of copper porphyrazines to G-quadruplex DNA is stronger than copper phthalocyanine, and their binding is favorable thermodynamically. Porphyrazines have potential to be used as anti-cancer compounds and are suitable complexes for pharmaceutical studies.
Materials and Methods: In the present experimental study, interaction of an anionic water-soluble phthalocyanine Cu(PcTs) and two cationic water-soluble tetrapyridinoporphyrazines, including [Cu(2,3-tmtppa)] 4+ and [Cu(3,4-tmtppa)]4+ complexes with human telomeric G-quadruplex
Findings: Fluorescent intercalator displacement indicated the displacement ability of the complexes with thiazole orange. Stern-Volmer plots of the porphyrazines exhibited a slight positive deviation from a straight line, suggesting both static and dynamic quenching. In addition, the quenching effect of the two porphyrazines was noticeably higher than the phthalocyanine, implying binding of Cu(PcTs) to both forms of the quadruplex was weaker compared to Cu(2,3-tmtppa) and Cu(3,4-tmtppa). Gibbs free energy (∆G) for binding was negative, implying that the interaction between the complexes and the G-quadruplex DNA was favorable thermodynamically.
Conclusion: The binding of copper porphyrazines to G-quadruplex DNA is stronger than copper phthalocyanine, and their binding is favorable thermodynamically. Porphyrazines have potential to be used as anti-cancer compounds and are suitable complexes for pharmaceutical studies.
Article Type: Original Manuscript |
Subject:
Agricultural Biotechnology
Received: 2016/07/19 | Accepted: 2017/08/20 | Published: 2018/09/22
Received: 2016/07/19 | Accepted: 2017/08/20 | Published: 2018/09/22
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