Volume 9, Issue 4 (2018)
JMBS 2018, 9(4): 635-641 |
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Zamani F, Merati A, Latifi M, Ghanbari Alanagh H, Nadipour F. Effect of Plasma Treatment on Cell Culture in Poly Lactic Glycolic Acid Nanofibrous Scaffold. JMBS 2018; 9 (4) :635-641
URL: http://biot.modares.ac.ir/article-22-14426-en.html
URL: http://biot.modares.ac.ir/article-22-14426-en.html
1- Hazrat-e Masoumeh University, Qom, Iran
2- “Advanced Textile Materials & Technologies Research Institute” and “Nano-fibrous Structures Department, Textile Engineering Faculty”, Amirkabir University of Technology, Tehran, Iran, Textile Engineering Faculty, Amirkabir University of Technology, NO. 424, Hafez Street, Tehran, Iran. Postal Code: 1591634311 , merati@aut.ac.ir
3- Nano-fibrous Structures Department, Textile Engineering Faculty, Amirkabir University of Technology, Tehran, Iran
4- Medical Nanotechnology Department, Advanced Technologies in Medicine Faculty, Tehran University of Medical Science, Tehran, Iran
2- “Advanced Textile Materials & Technologies Research Institute” and “Nano-fibrous Structures Department, Textile Engineering Faculty”, Amirkabir University of Technology, Tehran, Iran, Textile Engineering Faculty, Amirkabir University of Technology, NO. 424, Hafez Street, Tehran, Iran. Postal Code: 1591634311 , merati@aut.ac.ir
3- Nano-fibrous Structures Department, Textile Engineering Faculty, Amirkabir University of Technology, Tehran, Iran
4- Medical Nanotechnology Department, Advanced Technologies in Medicine Faculty, Tehran University of Medical Science, Tehran, Iran
Abstract: (3400 Views)
Aims: Tissue engineering and replacement of damaged tissue in medical science is very important and more effective than person-to-person transplantation. Therefore, the production of scaffolds from natural and synthetic polymers with desirable properties to reproduce damaged tissues is increasing. The aim of the present study was to investigate the effect of plasma treatment on contact angle or hydrophilicity of poly-lactic glycolic acid nanofibrous scaffolds and cell culture efficiency.
Materials and Methods: In the present experimental research, two types of solvents such as pure chloroform and the choloroform80% and dimethyl formaldehyde20% were used for electrospinning solution. The level of electrospun scaffolds was corrected by plasma technology; then, the African green monkey kidney (VERO) cells were cultured on them. The raw or non-treated electrospun scaffold was compared with that of plasma treated in hydrophilicity and cell culture viewpoints. To compare the hydrophilicity of scaffolds, the contact angle of them was measured.
Findings: The samples treated with plasma show lower contact angle and consequently higher hydrophilicity. C=O and C-O groups increased in the plasma-treated samples in comparison with those of raw samples. Plasma scaffold level correction improved the adhesion, growth, and proliferation of cells compared to non-treated scaffolds.
Conclusion: The contact angle of the plasma-treated samples is significantly reduced. Plasma treatment can increase the hydrophilicity of poly-lactic glycolic acid nanofibrous scaffolds, and cell adhesion and growth on plasma-treated scaffolds is better than cell growth and proliferation on non-treated scaffolds.
Materials and Methods: In the present experimental research, two types of solvents such as pure chloroform and the choloroform80% and dimethyl formaldehyde20% were used for electrospinning solution. The level of electrospun scaffolds was corrected by plasma technology; then, the African green monkey kidney (VERO) cells were cultured on them. The raw or non-treated electrospun scaffold was compared with that of plasma treated in hydrophilicity and cell culture viewpoints. To compare the hydrophilicity of scaffolds, the contact angle of them was measured.
Findings: The samples treated with plasma show lower contact angle and consequently higher hydrophilicity. C=O and C-O groups increased in the plasma-treated samples in comparison with those of raw samples. Plasma scaffold level correction improved the adhesion, growth, and proliferation of cells compared to non-treated scaffolds.
Conclusion: The contact angle of the plasma-treated samples is significantly reduced. Plasma treatment can increase the hydrophilicity of poly-lactic glycolic acid nanofibrous scaffolds, and cell adhesion and growth on plasma-treated scaffolds is better than cell growth and proliferation on non-treated scaffolds.
Article Type: _ |
Subject:
Agricultural Biotechnology
Received: 2017/01/22 | Accepted: 2017/09/6 | Published: 2018/12/21
Received: 2017/01/22 | Accepted: 2017/09/6 | Published: 2018/12/21
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