Volume 10, Issue 4 (2019)
JMBS 2019, 10(4): 527-534 |
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Mehrban S, Bahreini M, Asoodeh A, Korozhdehi B. Identification of native isolation, FUM1, isolated from poultry farm of Mashhad countryside and improving its keratinolytic activity by optimizing of environmental conditions using one factor at a time methodology. JMBS 2019; 10 (4) :527-534
URL: http://biot.modares.ac.ir/article-22-16166-en.html
URL: http://biot.modares.ac.ir/article-22-16166-en.html
1- Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
2- Department of Chemistry, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
3- Agricultural Biotechnology Department, Agriculture Faculty, Agriculture & Natural Resources College, University of Tehran, Karaj, Iran
2- Department of Chemistry, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
3- Agricultural Biotechnology Department, Agriculture Faculty, Agriculture & Natural Resources College, University of Tehran, Karaj, Iran
Abstract: (6022 Views)
Aims: Significant amounts of waste, including feathers, bones, blood, etc. are yearly produced by the poultry industry. Feathers are composed of 90% keratin protein, and the rest is composed of lipids and water. Keratinases are one of the most diverse and usable enzymes, which can be produced by bacterial and fungal microorganisms. These enzymes show a wide range of application in various fields.
Materials and Methods: In this study, the keratinolytic activity of the isolated strain from a poultry farm in Mashhad was evaluated and then the medium conditions for keratinase production were optimized. The strains were identified based on the morphological and biochemical methods. 16SrRNA gene of the strain was amplified by PCR and then sequenced. The strain proteolytic activity was examined and compared with its keratinolytic activity. Finally, strain growth ability tested in variety substrate.
Findings: Using 16SrRNA gene sequencing, morphological and biochemical identification, the strain shared 99.9% similarity with Bacillus mojavensis. Optimization of various factors, including temperature, pH, incubation time, carbon and nitrogen sources, aeration and inoculum size showed that the isolated strain has the highest keratinolytic activity at 37°C, 48 hour incubation period, pH=9.5, sucrose 1%, 3% substrate, aeration 75% and 6% (v/v) inoculum amount. None of the nitrogen sources had a positive effect.
Conclusion: The FUM-1 keratinolytic activity was increased approximately 3.38 fold by condition optimization of the medium, indicating the importance of environmental conditions. In the study, the strain with high keratinolytic activity was suggesting its potential use in biotechnological.
Materials and Methods: In this study, the keratinolytic activity of the isolated strain from a poultry farm in Mashhad was evaluated and then the medium conditions for keratinase production were optimized. The strains were identified based on the morphological and biochemical methods. 16SrRNA gene of the strain was amplified by PCR and then sequenced. The strain proteolytic activity was examined and compared with its keratinolytic activity. Finally, strain growth ability tested in variety substrate.
Findings: Using 16SrRNA gene sequencing, morphological and biochemical identification, the strain shared 99.9% similarity with Bacillus mojavensis. Optimization of various factors, including temperature, pH, incubation time, carbon and nitrogen sources, aeration and inoculum size showed that the isolated strain has the highest keratinolytic activity at 37°C, 48 hour incubation period, pH=9.5, sucrose 1%, 3% substrate, aeration 75% and 6% (v/v) inoculum amount. None of the nitrogen sources had a positive effect.
Conclusion: The FUM-1 keratinolytic activity was increased approximately 3.38 fold by condition optimization of the medium, indicating the importance of environmental conditions. In the study, the strain with high keratinolytic activity was suggesting its potential use in biotechnological.
Article Type: Research Paper |
Subject:
Agricultural Biotechnology
Received: 2017/11/20 | Accepted: 2019/05/15 | Published: 2019/12/21
Received: 2017/11/20 | Accepted: 2019/05/15 | Published: 2019/12/21
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