Volume 9, Issue 2 (2018)
JMBS 2018, 9(2): 301-308 |
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Karimi E, Yazdian F, Rasekh B, Akhavan Sepahy A, Rashedi H, Sheykhha M, et al . Biodesulfurization of Dibenzothiophene by Rhodococcus erythropolis IGTS8 in the Presence of Magnetic Nanoparticles and Carbon Nanotubes Surface-modified Polyethylene Glycol. JMBS 2018; 9 (2) :301-308
URL: http://biot.modares.ac.ir/article-22-17094-en.html
URL: http://biot.modares.ac.ir/article-22-17094-en.html
E. Karimi1 
, F. Yazdian * 2, B. Rasekh3 , A. Akhavan Sepahy4 , H. Rashedi5 , M.H. Sheykhha6 , B.F. Haghiroalsadat7 , A.S. Hatamian1
, F. Yazdian * 2, B. Rasekh3 , A. Akhavan Sepahy4 , H. Rashedi5 , M.H. Sheykhha6 , B.F. Haghiroalsadat7 , A.S. Hatamian1
1- Life Science Engineering Department, New Sciences & Technologies Faculty, University of Tehran, Tehran, Iran
2- Life Science Engineering Department, New Sciences & Technologies Faculty, University of Tehran, Tehran, Iran, New Sciences & Technologies Faculty, North Kargar Street, Tehran, Iran. Postal Code: 1439957131 , yazdian@ut.ac.ir
3- Biotechnology Research Center, Research Institute of Petroleum Industry, Tehran, Iran
4- Biology Department, Life Science Faculty, North Tehran Branch, Islamic Azad Universities, Tehran, Iran
5- Biotechnology Department, Chemical Engineering Faculty, University of Tehran, Tehran, Iran
6- Biotechnology Research Center, International Campus, Shahid Sadoughi University of Medical Sciences & Health Services, Yazd, Iran
7- New Medical Sciences & Technologies Department, Paramedical Faculty, Shahid Sadoughi University of Medical Sciences & Health Services, Yazd, Iran
2- Life Science Engineering Department, New Sciences & Technologies Faculty, University of Tehran, Tehran, Iran, New Sciences & Technologies Faculty, North Kargar Street, Tehran, Iran. Postal Code: 1439957131 , yazdian@ut.ac.ir
3- Biotechnology Research Center, Research Institute of Petroleum Industry, Tehran, Iran
4- Biology Department, Life Science Faculty, North Tehran Branch, Islamic Azad Universities, Tehran, Iran
5- Biotechnology Department, Chemical Engineering Faculty, University of Tehran, Tehran, Iran
6- Biotechnology Research Center, International Campus, Shahid Sadoughi University of Medical Sciences & Health Services, Yazd, Iran
7- New Medical Sciences & Technologies Department, Paramedical Faculty, Shahid Sadoughi University of Medical Sciences & Health Services, Yazd, Iran
Abstract: (4297 Views)
Aims: Today, crude oil is one of the main sources of energy. The combustion of sulfur-containing compounds in fossil fuels leads to the production of sulfur oxides that has adverse effects to human health and the environment. At the moment, the current method for removal of sulfur is Hydrodesulfurization. The aim of this study was to investigate the effect of magnetic nanoparticles and modified carbon nanotubes as nano-adsorbent on improving the biodesulfurization activity of microorganisms (Rhodococcus erythropolis IGTS8).
Materials and Methods: In the current experimental study, the nanoparticles were synthesized by chemical co-precipitation and the carbon nanotubes were initially carboxylated. Multi-layer carbon nanotubes were mixed with 95% sulfuric acid and 52% nitric acid (volume ratio 1:3) and, then, modified by polyethylene glycol. In order to characterize nanoparticles and carbon nanotubes, scanning transmission electron microscopy (STEM), X-ray diffraction, magnetic surveys, analysis of arch Raman, and fourier-transform infrared spectroscopy (FTIR) were conducted.
Findings: The size of nanoparticles was estimated to be 7-8nm and modified carbon nanotubes showed the highest solubility as well as stability in deionized water for two weeks. The growth of microorganisms in the presence of magnetic nanoparticles and carbon nanotubes compared to their absence increased by 40% and 8%, respectively. Moreover the desulfurization activity of microorganisms in the presence of magnetic nanoparticles and carbon nanotubes showed a significant increase compared to their absence.
Conclusion: Nanoparticles with adsorption of sulfur compounds increase their availability for microorganisms.
Materials and Methods: In the current experimental study, the nanoparticles were synthesized by chemical co-precipitation and the carbon nanotubes were initially carboxylated. Multi-layer carbon nanotubes were mixed with 95% sulfuric acid and 52% nitric acid (volume ratio 1:3) and, then, modified by polyethylene glycol. In order to characterize nanoparticles and carbon nanotubes, scanning transmission electron microscopy (STEM), X-ray diffraction, magnetic surveys, analysis of arch Raman, and fourier-transform infrared spectroscopy (FTIR) were conducted.
Findings: The size of nanoparticles was estimated to be 7-8nm and modified carbon nanotubes showed the highest solubility as well as stability in deionized water for two weeks. The growth of microorganisms in the presence of magnetic nanoparticles and carbon nanotubes compared to their absence increased by 40% and 8%, respectively. Moreover the desulfurization activity of microorganisms in the presence of magnetic nanoparticles and carbon nanotubes showed a significant increase compared to their absence.
Conclusion: Nanoparticles with adsorption of sulfur compounds increase their availability for microorganisms.
Article Type: Research Paper |
Subject:
Agricultural Biotechnology
Received: 2016/10/28 | Accepted: 2017/06/14 | Published: 2018/06/21
Received: 2016/10/28 | Accepted: 2017/06/14 | Published: 2018/06/21
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