Volume 10, Issue 4 (2019)
JMBS 2019, 10(4): 635-646 |
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Bakhshian Nik A, Vahidi B. Simulation of the Effects of Shear Flow of the Culture Medium Fluid on Stem Cells using the Scaffolds of Hard Tissue Engineering. JMBS 2019; 10 (4) :635-646
URL: http://biot.modares.ac.ir/article-22-21919-en.html
URL: http://biot.modares.ac.ir/article-22-21919-en.html
1- Life Science Engineering Department, New Sciences and Technologies Faculty, University of Tehran, Tehran, Iran
2- Life Science Engineering Department, New Sciences and Technologies Faculty, University of Tehran, Tehran, Iran, Science & Technology Faculty, North Kargar Street, Tehran, Iran. Postal Code: 1439957131 , bahman.vahidi@ut.ac.ir
2- Life Science Engineering Department, New Sciences and Technologies Faculty, University of Tehran, Tehran, Iran, Science & Technology Faculty, North Kargar Street, Tehran, Iran. Postal Code: 1439957131 , bahman.vahidi@ut.ac.ir
Abstract: (4029 Views)
Aims: In bone tissue engineering, the scaffold as a supportive structure, plays a vital role. Putting the scaffold in dynamic cell culture, such as perfusion bioreactor, makes the role of mechanical parameters such as shear stress and hydrodynamic pressure more important. On the other hand, these mechanical parameters are influenced by scaffold architecture. In this study, the effects of bone scaffold architecture on mechanical stimuli have been analyzed and their effects on the mesenchymal stem cell fate have been predicted.
Material & Methods: Using the tools of computer simulation, five bone scaffolds (Gyroid, high porous Gyroid, Diamond, IWP, and gradient architecture Gyroid) based on mathematical functions of minimal surfaces were designed and exposed in a simulated dynamic cell culture under the inlet velocities of 1, 10, 25, 50, and 100μm/s. Cell accumulation on the inner part of the scaffold was considered as an 8.5-micron layer. This layer was designed for Gyroid and IWP scaffolds.
Findings: Based on the results, Diamond scaffold showed the most efficient performance from the homogeneity of stresses point of view. In the presence of the cell layer, the von Mises stress was reported as 60 and 50 mPa on the Gyroid and IWP scaffolds, respectively which eases osteogenic differentiation.
Conclusion: In gradient architecture scaffolds under dynamic conditions, there is a gradient in shear stress that causes various signaling in different positions of theses scaffold and facilitates multi-differentiation of the cells on the same scaffold.
Material & Methods: Using the tools of computer simulation, five bone scaffolds (Gyroid, high porous Gyroid, Diamond, IWP, and gradient architecture Gyroid) based on mathematical functions of minimal surfaces were designed and exposed in a simulated dynamic cell culture under the inlet velocities of 1, 10, 25, 50, and 100μm/s. Cell accumulation on the inner part of the scaffold was considered as an 8.5-micron layer. This layer was designed for Gyroid and IWP scaffolds.
Findings: Based on the results, Diamond scaffold showed the most efficient performance from the homogeneity of stresses point of view. In the presence of the cell layer, the von Mises stress was reported as 60 and 50 mPa on the Gyroid and IWP scaffolds, respectively which eases osteogenic differentiation.
Conclusion: In gradient architecture scaffolds under dynamic conditions, there is a gradient in shear stress that causes various signaling in different positions of theses scaffold and facilitates multi-differentiation of the cells on the same scaffold.
Keywords: Bone Tissue Engineering, Mechanical Enviroment, Stem Cell, Scaffold, Shear Stress, Computational Simulation
Article Type: Original Research |
Subject:
Biological system
Received: 2018/06/26 | Accepted: 2019/06/12 | Published: 2019/12/21
Received: 2018/06/26 | Accepted: 2019/06/12 | Published: 2019/12/21
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