Volume 10, Issue 3 (2019)
JMBS 2019, 10(3): 465-471 |
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Zaghian S, Tohidi Moghadam T, Behmanesh M. Toxicity of Peptide-Functionalized Gold Nanorods in Hela Cell Line. JMBS 2019; 10 (3) :465-471
URL: http://biot.modares.ac.ir/article-22-25413-en.html
URL: http://biot.modares.ac.ir/article-22-25413-en.html
1- Nanobiotechnology Department, Biological Sciences Faculty, Tarbiat Modares University, Tehran, Iran
2- Nanobiotechnology Department, Biological Sciences Faculty, Tarbiat Modares University, Tehran, Iran, Tarbiat Modares University, Nasr Bridge, Jalal-Al-Ahmad Highway, Tehran, Iran. , behmanesh@modares.ac.ir
2- Nanobiotechnology Department, Biological Sciences Faculty, Tarbiat Modares University, Tehran, Iran, Tarbiat Modares University, Nasr Bridge, Jalal-Al-Ahmad Highway, Tehran, Iran. , behmanesh@modares.ac.ir
Abstract: (3702 Views)
The unique physicochemical properties of nanoscale plasmonic materials have attracted considerable attention in the fabrication of hybrid nano-bio structures because of their promising applications in biosensing, imaging, and controlled-release drug delivery. The purpose of this study was the synthesis of functionalized gold nanorods (GNRs) to both reduce the toxicity and increase the biocompatibility for further applications such as the design of a therapeutic nanocarrier for nucleic acid delivery to cancerous cells. In this study, GNRs were prepared by seed-mediated method and their surface was modified by polystyrene sulfonate (PSS) polymer. Then, peptide-functionalized GNRs was fabricated via ligand exchange method through the Au-S bond. The CTAB-GNRs and functionalized nanostructures were characterized using ultraviolet-visible spectrophotometry, transmission electron microscopy (TEM), and zeta potential measurement. Finally, the cytotoxicity effects of functionalized GNRs on Hela cells were studied by MTT assay. The optimal concentration of PSS and peptide, which did not cause any aggregation and morphological perturbations of the nanostructure were obtained 50μM and 1mM respectively. The survival percentage of treated Hela cells significantly increased by surface modification of GNRs with PSS and functionalization with peptide compared to CTAB-GNRs. While LC50 of functionalized GNRs was calculated 50nM, treated cells with the same concentrations of CTABGNRs survived less than 20%. Functionalization of GNRs increases its biocompatibility and improves applications of this nanostructure as a therapeutic carrier in cancerous cells.
Article Type: Brief Communication |
Subject:
Nanotechnology
Received: 2018/09/24 | Accepted: 2018/11/11 | Published: 2019/09/21
Received: 2018/09/24 | Accepted: 2018/11/11 | Published: 2019/09/21
References
1. Alkilany AM, Thompson LB, Boulos SP, Sisco PN, Murphy CJ. Gold nanorods: their potential for photothermal therapeutics and drug delivery, tempered by the complexity of their biological interactions. Adv Drug Deliv Rev. 2012;64(2):190-9. [Link] [DOI:10.1016/j.addr.2011.03.005]
2. Xia Y. Nanomaterials at work in biomedical research. Nat Mater. 2008;7:758-60. [Link] [DOI:10.1038/nmat2277]
3. Lei Y, Tang L, Xie Y, Xianyu Y, Zhang L, Wang P, et al. Gold nanoclusters-assisted delivery of NGF siRNA for effective treatment of pancreatic cancer. Nat Commun. 2017;8:15130. [Link] [DOI:10.1038/ncomms15130]
4. Gui C, Cui DX. Functionalized gold nanorods for tumor imaging and targeted therapy. Cancer Biol Med. 2012;9(4):221-33. [Link]
5. Chen QR, Zhang L, Stass SA, Mixson AJ. Branched co-polymers of histidine and lysine are efficient carriers of plasmids. Nucleic Acids Res. 2001;29(6):1334-40. [Link] [DOI:10.1093/nar/29.6.1334]
6. Adura C, Guerrero S, Salas E, Medel L, Riveros A, Mena J, et al. Stable conjugates of peptides with gold nanorods for biomedical applications with reduced effects on cell viability. ACS Appl Mater Interfaces. 2013;5(10):4076-85. [Link] [DOI:10.1021/am3028537]
7. Selvakannan PR, Mandal S, Phadtare S, Pasricha R, Sastry M. Capping of gold nanoparticles by the amino acid lysine renders them water-dispersible. Langmuir. 2003;19(8):3545-9. [Link] [DOI:10.1021/la026906v]
8. Hamon C, Bizien T, Artzner F, Even-Hernandez P, Marchi V. Replacement of CTAB with peptidic ligands at the surface of gold nanorods and their self-assembling properties. J Colloid Interface Sci. 2014;424:90-7. [Link] [DOI:10.1016/j.jcis.2014.03.002]
9. Alkilany AM, Nagaria PK, Wyatt MD, Murphy CJ. Cation exchange on the surface of gold nanorods with a polymerizable surfactant: polymerization, stability, and toxicity evaluation. Langmuir. 2010;26(12):9328-33. [Link] [DOI:10.1021/la100253k]
10. Connor EE, Mwamuka J, Gole A, Murphy CJ, Wyatt MD. Gold nanoparticles are taken up by human cells but do not cause acute cytotoxicity. Small. 2005;1(3):325-7. [Link] [DOI:10.1002/smll.200400093]
11. Chen QR, Zhang L, Stass SA, Mixson AJ. Co-polymer of histidine and lysine markedly enhances transfection efficiency of liposomes. Gene Ther. 2000;7(19):1698-705. [Link] [DOI:10.1038/sj.gt.3301294]
12. Tohidi Moghadam T, Ranjbar B. Heat induced aggregation of gold nanorods for rapid visual detection of lysozyme. Talanta. 2015;144:778-87. [Link] [DOI:10.1016/j.talanta.2015.06.025]
13. Becker R, Liedberg B, Käll PO. CTAB promoted synthesis of Au nanorods - Temperature effects and stability considerations. J Colloid Interface Sci. 2010;343(1):25-30. [Link] [DOI:10.1016/j.jcis.2009.10.075]
14. Kang, SK, Chah S, Yun CY, Yi J. Aspect ratio controlled synthesis of gold nanorods. Korean J Chem Eng. 2003;20(6):1145-8. [Link] [DOI:10.1007/BF02706952]
15. El-Brolossy TA, Abdallah T, Mohamed MB, Abdallah S, Easawi K, Negm S, Talaat H. Shape and size dependence of the surface plasmon resonance of gold nanoparticles studied by Photoacoustic technique. Eur Phys J Spec Top. 2008;153(1):361-4. [Link] [DOI:10.1140/epjst/e2008-00462-0]
16. Ni W, Kou X, Yang Z, Wang J. Tailoring longitudinal surface plasmon wavelengths, scattering and absorption cross sections of gold nanorods. ACS Nano. 2008;2(4):677-86. [Link] [DOI:10.1021/nn7003603]
17. Murphy CJ, Gole AM, Stone JW, Sisco PN, Alkilany AM, Goldsmith EC, et al. Gold nanoparticles in biology: beyond toxicity to cellular imaging. Acc Chem Res. 2008;41(12):1721-30. [Link] [DOI:10.1021/ar800035u]
18. Boca SC, Astilean S. Detoxification of gold nanorods by conjugation with thiolated poly(ethylene glycol) and their assessment as SERS-active carriers of Raman tags. Nanotechnology. 2010;21(23):235601. [Link] [DOI:10.1088/0957-4484/21/23/235601]
19. Rayavarapu RG, Petersen W, Hartsuiker L, Chin P, Janssen H, van Leeuwen FW, et al. In vitro toxicity studies of polymer-coated gold nanorods. Nanotechnology. 2010;21(14):145101. [Link] [DOI:10.1088/0957-4484/21/14/145101]
20. Leonov AP, Zheng J, Clogston JD, Stern ST, Patri AK, Wei A. Detoxification of Gold Nanorods by Treatment with Polystyrenesulfonate. ACS Nano. 2008;2(12):2481-8. [Link] [DOI:10.1021/nn800466c]
21. Wan J, Wang JH, Liu T, Xie Z, Yu XF, Li W. Surface chemistry but not aspect ratio mediates the biological toxicity of gold nanorods in vitro and in vivo. Sci Rep. 2015;5:11398. [Link] [DOI:10.1038/srep11398]
22. Hansen MB, Nielsen SE, Berg K. Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill. J Immunol Methods. 1989;119(2):203-10. [Link] [DOI:10.1016/0022-1759(89)90397-9]
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