1- Department of Anaerobic Bacterial Vaccine Research and Production, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj , esmaeilnejadp@gmail.com
2- Department of Anaerobic Bacterial Vaccine Research and Production, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj
2- Department of Anaerobic Bacterial Vaccine Research and Production, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj
Abstract: (253 Views)
The process of diphtheria toxoid production was designed by using SuperPro Designer and the effect of the applied changes in process on the yield and costs of the manufacturing was investigated. First, giving the information of the real process of the toxoid production, a bioreactor with improved operational conditions and a disc stack centrifuge instead of the filter press, which is applied for the bacterial debris separation, were utilized. Such alterations followed the addition of a pump between the bioreactor and centrifuge. The results indicated that improvement of the bioreactor operational conditions can lead to the 25% increase in the toxin production, i.e., the increase of toxoid production from 7,000,000 doses to 8,750,000 doses. The toxin waste through filter press (14%) may be remarkably reduced by using the centrifuge, which in turn resulted in the 44% enhancement in the toxoid production. Such alterations can result in the 16% reduction in the separation operation time, 29% reduction in water consumption and 32% increase in the energy consumption. Overall, the simulation results showed that the costs of the new equipment suggested to be used in the improved process can be recoverable through running two batches.
Article Type: Original Research |
Subject:
Industrial Biotechnology
Received: 2022/01/24 | Accepted: 2022/11/27
Received: 2022/01/24 | Accepted: 2022/11/27
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