1- University of Kashan
2- university of Kashan ,mansournia@kashanu.ac.ir
2- university of Kashan ,
Abstract: (38 Views)
The aim of present research is development of a simple green approach to produce Ag/AgCl nanocomposites using bacterial strains Bacillus haynesii and Bacillus halotorans, nominated at PN14F and B3, respectively, via an extracellular process. The bacterial strains PN14F and B3 were isolated from the soil and wastewater samples, using dilution and direct cultivation method. The Ag/AgCl nanocomposites were synthesized from the reaction of silver(I) nitrate solution and supernatant under completely sterile conditions in the presence of light. Moreover, a series of controlled experiments were provided to optimize some reaction conditions such as substrate concentration, PH, substrate volume, bacterial volume, the presence of glucose as an electron donor and silver(I) nitrate as inducer. The products were characterized using various techniques such as UV-Vis, XRD, FT-IR, FE-SEM and EDX. The resulting bionanocomposites (Ag1 and Ag2), with an average particle size of 30 and 22.3 nm, were efficient heterogeneous catalysts for reducing para-nitrophenol to para-aminophenl. Further, it was demonstrated their activity as the antibacterial properties against gram positive and negative bacteria. The results showed that the Ag2 sample with reducing time of 15 min is a more efficient catalyst than the Ag1 nanocomposite, which can be attributed to the smaller size of the Ag2 nanoparticles.
Keywords: Bionanocomposite, Ag/AgCl, Extracellular process, Heterogeneous catalyst, Antimicrobial activity
Article Type: Original Research |
Subject:
Microbial biotechnology
Received: 2023/01/25 | Accepted: 2024/06/8
Received: 2023/01/25 | Accepted: 2024/06/8
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