Volume 8, Issue 2 (2017)
JMBS 2017, 8(2): 140-150 |
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Immunization against New castle disease by DNA vaccine in SPF chicken. JMBS 2017; 8 (2) :140-150
URL: http://biot.modares.ac.ir/article-22-4453-en.html
URL: http://biot.modares.ac.ir/article-22-4453-en.html
Abstract: (5875 Views)
Newcastle disease is a fatal viral disease which is highly contagious that affects most species of birds and is a major economic threat in the poultry industry. Both the HN and F glycoproteins of Newcastle disease virus (NDV) are essential for pathogenicity and virus infectivity. This study describes immunization of DNA vaccines encoding the HN, F or both the genes of New castle disease virus. In our previous study, the antigen expression of the insert genes has been validated in vitro by Western Blotting and Indirect Immunosenest. In this study, ELISA and HI analysis of the in vivo experiment on SPF (specific Pathogen Free) chickens showed the induction of humoral responses by the DNA vaccines. Our finding indicated that twice vaccination with pDNA was able to elicit significant antibody titers (P< 0.05) by either monocistronic (pIRES/HN and pIRES/F) or bicistronic (pIRES/F/HN) plasmid, after one week of second pDNA vaccination (booster). The results proposed that DNA immunization of chickens at second vaccination had enhanced the antibody response successfully. Also, it revealed that vaccination with the co-expression plasmid pIRES/HN/F can induce a stronger antibody response than vaccination with pIRES/HN or pIRES/F alone.
Article Type: Original Manuscript |
Subject:
Agricultural Biotechnology
Received: 2016/03/3 | Accepted: 2017/09/23 | Published: 2018/01/27
Received: 2016/03/3 | Accepted: 2017/09/23 | Published: 2018/01/27
References
1. Alexander D., Newcastle disease and other avian Paramyxoviridae infection, In Diseases of Poultry, 10th ed, p 541–569.
2. Payal Arora, B D Lakhchaura, S K Garg. (2010) Evaluaion of immunogenic potential of 75kDa and 56kDa proteins of newcastle disease virus (NDV). Indian J of Exp Biol. 48, 889-895.
3. Krishnamurthy S, Huang Z, and Samal SK. (2000) Recovery of a virulent strain of Newcastle disease virus from cloned cDNA: expression of a foreign gene results in growth retardation and attenuation. Virology. 278, 168-182
4. Morgan RW, Gelb Jr J, Schreurs CS, Lütticken D, Rosenberger JK and Sondermeijer PJ. (1992) Protection of chickens from Newcastle and Marek's diseases with a recombinant herpesvirus of turkeys vaccine expressing the Newcastle disease virus fusion protein. Avian Dis. 858-870
5. Nagai Y, Klenk H-D and Rott R. (1976) Proteolytic cleavage of the viral glycoproteins and its significance for the virulence of Newcastle disease virus. Virology. 72, 494-508
6. Gurunathan S, Klinman DM, and Seder RA. (2000) DNA vaccines: immunology, application, and optimization. Immunol. 18, 927-974.
7. Vijayarani K, Kumanan K, Albert A, Padmanaban VD. (1993) Immunogenicity of Newcastle virus subunits. Indian J Viro. 9,9
8. Loke C, Omar AR, Raha A, and Yusoff K. (2005) Improved protection from velogenic Newcastle disease virus challenge following multiple immunizations with plasmid DNA encoding for F and HN genes. Vet. Immunol. Immunopathol. 106, 259-267
9. Dortmans JCFM, Guus K, Peter JMR, and Peeters BPH. (2011) Virulence of Newcastle disease virus: what is know so far?J. Vet. Res. 122, 2-11
10. Allan W and Gough, R. (1974) A standard haemagglutination inhibition test for Newcastle disease.(1). Vet. Rec 1997, 95,120-123.
11. Stone-Hulslander J and Morrison TG. (1997) Detection of an interaction between the HN and F proteins in Newcastle disease virus-infected cells. J. Virol. 71, 6287-6295
12. Reynolds D and Maraqa A. (2000) Protective immunity against Newcastle disease: the role of antibodies specific to Newcastle disease virus polypeptides. Avian Dis. 138-144
13. Payal Arora, B D Lakhchaura, S K Garg. (2010) Evaluaion of immunogenic potential of 75kDa and 56kDa proteins of newcastle disease virus (NDV). Indian J of Exp Biol. 48, 889-895
14. Donnelly JJ, Wahren B, and Liu MA. (2005) DNA vaccines: progress and challenges. J. Immunol. 175, 633-639.
15. Reynolds D and Maraqa A. (2000) Protective immunity against Newcastle disease: the role of cell-mediated immunity. Avian Dis. 145-154.
16. Reddy GS & Srinivasan VA. (1992) Use of BHK cell culture adapted Newcastle disease virus for immunization of chicks. Vaccine. 10(3),164-6.
17. Sakaguchi M, Nakamura H, Sonoda K, Hamada F and Hirai K. (1996) Protection of chickens from Newcastle disease by vaccination with a linear plasmid DNA expressing the F protein of Newcastle disease virus. Vaccine. 14, 747-752
18. Vijayarani K, Kumanan K, Albert A, Padmanaban VD. (1993) Immunogenicity of Newcastle virus subunits. Indian J Viro. 9,9
19. Jazayeri SD, Ideris A, Zakaria Z, Shameli K, Moeini H, and Omar AR. (2012) Cytotoxicity and immunological responses following oral vaccination of nanoencapsulated avian influenza virus H5 DNA vaccine with green synthesis silver nanoparticles. Controlled Release. 161, 116-123
20. Pedersen N, Hansen S, Heydenreich AV, Kristensen HG and Poulsen HS. (2006) Solid lipid nanoparticles can effectively bind DNA, streptavidin and biotinylated ligands. Eur. J. Pharm. Biopharm. 62, 155-162
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