Volume 3, Issue 1 (2012)                   JMBS 2012, 3(1): 57-65 | Back to browse issues page

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Maleksabet N, nasiri khalili M A, masoumian M. Thermal stability of recombinant human interferon gamma produced in E.coli. JMBS 2012; 3 (1) :57-65
URL: http://biot.modares.ac.ir/article-22-7929-en.html
1- shahid babaee highway-lavizan-science & biotechnology institute, Tehran
2- Enghelab square-Tehran university- Institute of Biochemistry- Biophysics, Tehran
Abstract:   (10841 Views)
The stability of recombinant proteins has become an increasingly important as more protein therapeutics are developed.In this study, the stability of recombinant human interferon gamma was investigated under storage condition for 0-9 months after production time at 4 and 25℃. The evaluation of biological activity, covalent dimerization, deamidation and oxidation of protein was done by cell culture, HPLC and SDS- PAGE. The results showed represents that antiviral activity was not decreased at 4℃ but decreased as temperature increased to 25℃. The inormation rate of deamidated and oxidized forms and covalent dimers at 25℃ was more rapid than 4℃. Therefore, rhIFN-γ has high stability at 4℃ comparing to 25℃.
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Article Type: _ | Subject: biochemistry|Biotechnology
Received: 2011/10/12 | Accepted: 2012/01/31 | Published: 2012/11/18

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