Modares Journal of Biotechnology

Abstract Curcumin, a potent antioxidant extracted from the turmeric plant, is known for its ability to suppress reactive oxygen species (ROS) through activation of antioxidant response elements. However, its clinical utility is limited by poor solubility and rapid elimination. In this study, we aimed to enhance the solubility and bioavailability of curcumin by encapsulating it in fifth-generation polyamidoamine dendrimer nanoparticles. UV-Vis and fluorescence spectroscopy confirmed successful encapsulation, with an average nanoparticle size of 275 nm and a positive surface charge of 8 mV. The nanocarrier exhibited a loading capacity of approximately curcumin6 mol per dendrimer 1mol and achieved complete drug release within 72 hours. Cellular uptake assays using a live/dead assay revealed superior uptake of the nanocarrier compared to free curcumin, leading to a significant reduction in cellular ROS levels and apoptosis. Furthermore, in a model of induced oxidative stress using H2O2, curcumin-loaded dendrimer nanoparticles significantly reduced cellular ROS levels compared to free curcumin. These findings highlight the potential of this nanocarrier as an effective and intelligent system for delivering water-insoluble drugs, offering a promising approach for improving the therapeutic efficacy of curcumin.

Abstract Aims: The accumulation of free radicals in the body leads to damages to cellular biopolymers through oxidative stress. Due to the increasing proliferation of heavy metals in soil and water environments, finding efficient methods for diagnostic detection and measurement of heavy metals in contaminated environments is very important. Cell-based biosensors can produce a measurable signal in response to specific chemical or physical agent in their environment. In this study, stable hepatoma Huh7 ARE-reporter cell line was developed containing luciferase gene with the aim of monitoring lead toxicity. This biosensor is reported to be able to detect lead by expressed signal which is measurable. The luciferase assay and Real time-PCR were performed.
Materials and Methods: In this experimental research, the Huh7-1x-ARE-luc was stably transferred in to the Huh7 cells and transfected cells were selected. After 5 passages, stable clones were isolated to confirm plasmid entrance. Luciferase activity of the Huh7-1x-ARE-luc cell line was performed with 0-80μM lead concentration to induce oxidative stress response. Cell viability was assessed by MTT. With Real time PCR, AREKEAP1 pathway gene expression were detected. Statistical analysis was performed by ΔCt method, using graphpad prism 6 software.
Findings: The gene expression of the reporter gene increased with increasing oxidative stress. Reducing the expression of the reporter gene was observed after 30 μM. 35 μM lead inhibited 50% cell metabolism. Expression of antioxidant pathway genes was significantly increased in 30 μM leaded cells compared to control gene.
Conclusion: The biosensor prepared from Huh7-1x-ARE-luc cell line of the reporter gene can be a convenient and efficient means for measuring oxidative compounds such as heavy metals such as lead.

Abstract A high population of the sea anemone inhabit in the intertidal zone of the Hormoz Island. The tidal zones generally are characterized by severe environment stresses (vis, UV and high temperature), that can increase the intracellular oxidation and free radicals as well. Oxidation and free radicals is paved by the animal defensive mechanisms, including accumulation of antioxidant compounds and enzymes. In this study, carpet anemones sampled from intertidal zone of the eastern city of Hormoz Island (IRAN). Extraction was performed by using of PBS and methanol 40% solvents: 2 g dry weight of the mucous, by 2 replications, and 10 g wet weight of the oral plate, by 6 replications, from 6 samples. Different concentrations of extracts were made for antioxidant tests. The antioxidant properties of extract were measured by DPPH and FRC methods. Results showed that a correlation is between the antioxidant activity and the concentration of extracts in two methods. In DPPH assay, IC50 values was 1.469±0.208, 1.85±0.016 for mucus extract by PBS and methanol 40%, respectively, and it was 0.733±0.06, 0.444±0.036 mg/ml, for oral plate extract by PBS and methanol 40%. Our results showed that the antioxidant activity of oral plate was significantly higher than mucous in this anemone.

Abstract Oxidative Stress is fast becoming the nutritional and medical buzzword for the 21st century, and imPlicated in a growing list of diseases, from cataract to cancer. Oxidative stress occurs when the amount of free radicals in the body exceeds its pool of available antioxidants. Some researches indicate that margarine contain Trans fatty acids as major sources of dietrary free radicals and oxidative Stress. But other researches has been shown that because of supplementation of mairganie with some antioxidant such as alpha-carotene, beta-carotene, vitamin C and specially vitamin E, significantly decreased free radicals and oxidative stress and increased the levels of the supplied antioxidants in plasma. In this study, we examined the effect of The use fortified margarine on oxidative stress markers in young male, rats. To evaluate the margarine effect, 20 wistar rats were divided into 2 groups and selected as control and marganine groups. In second groups margarine were added to their chow as 15% (w/w). After 4 months blood samples were obtained, Then plasma levels of antioxidant, lipid peroxidation and thiol group of its, has been measured. This experiment showed that margarine intake has a positive effect on markers of oxidative stress, and consumption of antioxidant supplied in a full-fat margarine and consumed as a part of normal diet, effectively increases the blood levels of antioxidant and eliminats of free radicals.