Extracellular peroxidase enzyme production in native bacteria Bacillus tequilensis and determination of some biochemical properties of enzyme

Ahmadi, Fathollah; Abbasi, Alireza; Yakhchali, B.; Alizade, Hooshang; Pourbabaee ‎, A.A.

Extracellular peroxidase enzyme production in native bacteria Bacillus tequilensis and determination of some biochemical properties of enzyme

Document Type : Original Research

Authors

fathollah ahmadi ¹

Alireza abbasi ²

B. Yakhchali ³

Hooshang alizade ²

A.A. Pourbabaee ‎ ⁴

¹ Department of biotechnology, College of Agriculture & Natural Resources, University of Tehran

² Department of agronomy and plant breeding, College of Agricultural Science & Engineering, University of Tehran

³ National Institute of Genetic Engineering and Biotechnology

⁴ Department of Soil Sience Faculty of Agriculture Engineering and Technology College of Agriculture & Natural Resources, University of Tehran

Abstract

Aims: Peroxidases are used in a wide range of biotechnological processes, most of which are carried out at high temperatures and high pH levels. Since most of the commonly used peroxidases are unstable and inactive in alkaline conditions and high temprature, it is necessary to find thermoalkalophilic peroxidases for practical purposes.

Materials and Methods: In this study, extracellular production of peroxidase in the native strain Bacillus tequilensis was studied. for this purpose, Enzyme activity was evaluated using two substrates 2,4-DCP and pyrogallol in bacterial liquid culture and the effect of culture time on enzyme production, as well as the effect of parameters such as pH and temperature on enzyme activity investigated. The relative purification of the enzyme was performed using ion exchange chromatography with sephadex DEAE A50 and the kinetic parameters of enzyme activity were evaluated. In this study, kinetic parameters such as Km and Vmax were calculated.

Results: Measurement of enzyme activity at different times of culture indicated that the highest amount of peroxidase production was obtained 72 h after bacterial culture.

Keywords

Bacillus tequilensis

Peroxidase

thermoalkylphil

Kinetic parameters

pyrogallol

4-DCP

20.1001.1.23222115.1398.11.1.7.2

Subjects

Microbial biotechnology

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