Optimization of the production and extraction of phycocyanin from the Anabaena doliolum wet biomass

Bahrami, Asieh; Zolghadri, Samaneh; Deilami, Ehteram

Optimization of the production and extraction of phycocyanin from the Anabaena doliolum wet biomass

Document Type : Original Research

Authors

Asieh Bahrami ¹

Samaneh Zolghadri ²

Ehteram Deilami ¹

¹ Islamic Azad University

² Islamic Azad University, Jahrom Branch

Abstract

Abstract. Phycocyanine (PC) belongs to a group of protein receptor proteins called phycobiliprotein. All of the phycobiliprotein are multi-chain proteins made up of apoproteins. Which are covalently attached to the phyclobilins. This experimental study was carried out on strain of native Anabaena doliolum, Isolated from soils and waters of south Iran were Masjed Soleyman area. The cyanobacteria were grown and stored in BG11 medium. Then, the amount of phycocyanin produced under different light treatment and the amount of phycocyanin extracted using different ratios of multi-buffer and at two different temperatures were evaluated. The results of this study showed that the highest growth rate is when the sample is exposed to green light for three to five days. The best amount of extraction for distilled water and at a refrigerator temperature (0^◦C) with a ratio of 3:1 biomass/solvent is equal to 0.03 ± 15 µg/ml. Also, at the environment temperature, phosphate buffer is a more suitable solvent for extracting phycocyanine at a ratio of one to two with a value of 0.05 ± 8 µg/ml. In general, it can be said that the growth rate, pigment production and optimum extraction conditions for each species are quite different, and the optimal extraction of phycocyanin in a species is also dependent on various factors such as time, temperature, solvent and the ratio of biomass to solvent.

Keywords

phycobiliprotein

Cyanobacteria

microalgea

Anabaena doliolum

20.1001.1.23222115.1399.11.2.4.5

Subjects

Agricultural Biotechnology

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