Gene delivery to tobacco cells using single walled carbon nanotubes: a new generation of gene transfer vehicles

Golestanipour, Arezoo; Aalami, Ali; Nikkhah, Maryam; Hosseinkhani, Saman

Gene delivery to tobacco cells using single walled carbon nanotubes: a new generation of gene transfer vehicles

Document Type : Original Research

Authors

Arezoo Golestanipour ¹

Ali Aalami ²

Maryam Nikkhah ³

Saman Hosseinkhani ⁴

¹ Department of Plant Biotechnology, University Campus 2, University of Guilan, Rasht, Iran

² Department of Biotechnology, Faculty of Agricultural Sciences, University of Guilan,

³ Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran

⁴ Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran

Abstract

Developing a technique for efficient and safe gene delivery to plant cells is a fundamental aim of plant biotechnology. Agrobacterium mediated transformation as the most common and practical method in plant gene delivery has considerable difficulties such as limitation in applicable for plant species. In recent years several new methods have been suggested, although none of them could be a good replacement. The use of nanotechnology has been provided new solutions to overcome the limitations of biotechnology. Designing biocompatible nanostructures for passing cell barriers and targeted delivery of cargo has improved the biological achievements. In this research the capability of arginine functionalized single-walled carbon nanotube (Arg-SWNT) as a new carrier to transfer plasmid DNA, which codes green fluorescent protein (GFP) to tobacco suspension cells, has been investigation. It is suggested that single-walled carbon nanotubes can pass through cell wall pores and plasma membrane while it carries plasmid DNA along with. The fluorescence microscopy images illustrate the success of gene delivery by Arg-SWNT

Keywords

Arginine

Green fluorescent protein

Single-walled carbon nanotube

20.1001.1.23222115.1399.11.4.9.4

Subjects

Nanotechnology

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