Production optimization of canthaxanthin pigment from radioresistant Dietzia maris by response surface methodology and evaluation of its effect on cell culture

Salehi Bakhtiari, Atefeh; Etemadifar, Zahra; Borhani, Matia Sadat

Production optimization of canthaxanthin pigment from radioresistant Dietzia maris by response surface methodology and evaluation of its effect on cell culture

Document Type : Original Research

Authors

Atefeh Salehi Bakhtiari ¹

Zahra Etemadifar ¹

Matia Sadat Borhani ²

¹ University of Isfahan

² Biology Department, Faculty of Sciences, Gonbad Kavous University, Golestan, Iran

Abstract

Background: Carotenoids are biological antioxidants and play important roles in protecting the body from diseases and aging. Canthaxanthin is one of the most widely used carotenoids in the industry and medicine. This study aimed to investigate the biological properties of canthaxanthin pigment as well as its production optimization in a low-cost medium using a radioresistant microbial strain named Dietzia maris.

Materials and methods: Bacterial carotenoids were extracted and its antibacterial, anti-tumor, and cytotoxicity properties were investigated. Then, the effect of Krebs intermediates and pH on the production of pigment and microbial biomass in the whey medium was investigated using the response surface methodology.

Results: Maximum pigment production was found to be 92/54 mg/l in whey culture medium at pH 8 and in the presence of 12.5 mM of each of citrate, glutamate, malate, and succinate by the response surface method. The pigment did not show any cytotoxic effect on Hela, HFB, and MCF-7 cell lines. Besides, the pigment did not have any antibacterial properties.

Conclusion: Radioresistant microbial strains are better candidates for microbial pigment production due to their stability and high antioxidant activity. In this study, a whey culture medium was used to reduce the production cost of canthaxanthin. The addition of Krebs intermediaries in the fermentation medium increased the pigment production by Dietzia maris significantly.

Keywords

Dietzia maris

Carotenoids

Canthaxanthin

Radioresistant

Whey

Subjects

Microbial biotechnology

1. Sathasivam, R., and Ki, J. S. (2018). A review of the biological activities of microalgal carotenoids and their potential use in healthcare and cosmetic industries. Mar drugs, 16(1), 26.
2. Mortensen, A. (2006). Carotenoids and other pigments as natural colorants. Pure Appl chem, 78(8), 1477-1491.
3. Sandmann, G. (2001). Carotenoid biosynthesis and biotechnological application. Arch Biochem Biophys, 385(1), 4-12.
4. Rao, A. V., and Rao, L. G. (2007). Carotenoids and human health. Pharmacol res, 55(3), 207-216.
5. Yoshida, K., Inoue, K., Takahashi, Y., Ueda, S., Isoda, K., Yagi, K., and Maeda, I. (2008). Novel carotenoid-based biosensor for simple visual detection of arsenite: characterization and preliminary evaluation for environmental application. Appl. Environ. Microbiol. 74(21):6730-6738.
6. Vachali, P., Bhosale, P., and Bernstein, P. S. (2012). Microbial carotenoids. In Microbial carotenoids from fungi (pp. 41-59). Humana Press, Totowa, NJ.
7. Tian, B., and Hua, Y. (2010). Carotenoid biosynthesis in extremophilic Deinococcus–Thermus bacteria. Trends microbiol, 18(11), 512-520.
8. Nasrabadi, M. R. N., and Razavi, S. H. (2010). Use of response surface methodology in a fed-batch process for optimization of tricarboxylic acid cycle intermediates to achieve high levels of canthaxanthin from Dietzia natronolimnaea HS-1. J. Biosci. Bioeng, 109(4), 361-368.
9. Zamanian, N. and Etemadifar, Z. (2016). Isolation and molecular identification of a UV-resistant strain of Dietzia maris and antioxidant activity of pigment. Biological Journal of Microorganism. 5(18): 83-94.
10. Zamanian, S. N., and Etemadifar, Z. (2017). Radical scavengering of pigments from novel strains of Dietzia schimae and Microbacterium esteraromaticum. Progress in Biological Sciences, 6(2), 159-170.
11. Rodrigues, L. R., Teixeira, J. A., and Oliveira, R. (2006). Low-cost fermentative medium for biosurfactant production by probiotic bacteria. Biochem. Eng. J., 32(3), 135-142.
12. Howe, J. A., and Tanumihardjo, S. A. (2006). Evaluation of analytical methods for carotenoid extraction from biofortified maize (Zea mays sp.). J Agric Food Chem, 54(21), 7992-7997.
13. Nasri Nasrabadi, M. R., and Razavi, S. H. (2010). Enhancement of canthaxanthin production from Dietzia natronolimnaea HS-1 in a fed-batch process using trace elements and statistical methods. Braz J Chem Eng, 27(4), 517-529.
14. Marova, I., Carnecka, M., Halienova, A., Certik, M., Dvorakova, T., and Haronikova, A. (2012). Use of several waste substrates for carotenoid-rich yeast biomass production. J. Environ. Manage, 95, S338-S342.
15. Mohana, D., Thippeswamy, S., and Abhishe, R. (2013). Antioxidant, antibacterial, and ultraviolet-protective properties of carotenoids isolated from Micrococcus spp. Radiat Prot Environ, 36(4), 168-174.
16. Rezaeeyan, Z., Safarpour, A., Amoozegar, M. A., Babavalian, H., Tebyanian, H., and Shakeri, F. (2017). High carotenoid production by a halotolerant bacterium, Kocuria sp. strain QWT-12 and anticancer activity of its carotenoid. EXCLI journal, 16, 840.
17. Chen, D., Han, Y., and Gu, Z. (2006). Application of statistical methodology to the optimization of fermentative medium for carotenoids production by Rhodobacter sphaeroides. Process Biochem, 41(8), 1773-1778.
18. Panda, B.P., Ali, M., and Javed, S. (2007). Fermentation process optimization. Res. J. Microbiol, 2(3), 201-208.
19. Hamidi, M., Abdin, M. Z., Nazemyieh, H., Hejazi, M. A., and Hejazi, M. S. (2014). Optimization of total carotenoid production by Halorubrum Sp. TBZ126 using response surface methodology. J. Microb. Biochem. Technol, 6, 286-294.
20. El-Banna, A.A., El-Razek, A.M.A., El-Mahdy, A.R. (2012). Some factors affecting the production of carotenoids by Rhodotorula glutinis var. glutinis. Food Nutr Sci, 3(01), 64.
21. Khodaiyan, F., Razavi, S.H. and Mousavi, S.M. (2008). Optimization of canthaxanthin production by Dietzia natronolimnaea HS-1 from cheese whey using statistical experimental methods. Biochem. Eng. J., 40(3):415-422.
22. Bhosale, P. (2004). Environmental and cultural stimulants in the production of carotenoids from microorganisms. Appl Microbiol Biot, 63(4), 351-361.
23. An, G.H. (2001). Improved growth of the red yeast, Phaffia rhodozyma (Xanthophyllomyces dendrorhous), in the presence of tricarboxylic acid cycle intermediates. Biotechnol. Lett, 23(12), 1005-1009.
24. Matos, H.R., Mascio, P., and Medeiros, M.H. (2000). Protective effect of lycopene on lipid peroxidation and oxidative DNA damage in cell culture. Arch. Biochem. Biophys, 383(1): 56-59.
25. Geweely, N.S. (2011). Investigation of the optimum condition and antimicrobial activities of pigments from four potent pigment-producing fungal species. J. Life Sci, 5(9): 201.
26. Coppens, I. (2013). Targeting lipid biosynthesis and salvage in apicomplexan parasites for improved chemotherapies. Nat. Rev. Microbiol, 11(12): 823.