Novel strain Staphylococcus pasteuri sp. Ta-31 as a bioreactor for copper nanoparticles production

Kordzangeneh, Hadis; Jookar Kashi, Fereshteh

Novel strain Staphylococcus pasteuri sp. Ta-31 as a bioreactor for copper nanoparticles production

Document Type : Original Research

Authors

Hadis Kordzangeneh ¹

fereshteh Jookar kashi ²

¹ Biotechnology Division, Department of Molecular and Cell Biology, Faculty of Chemistry, University of Kashan, Kashan, Iran

² Department of Cell and Molecular Biology, Faculty of Chemistry, University of Kashan, Kashan, Iran

Abstract

This study aimed to isolate and identify bacteria from soils contaminated with copper and have access to a capable bacterial strain for producing copper nanoparticles (CuNPs). The present study showed the extracellular production of copper nanoparticles using strain Ta-31. The effect of various factors such as substrate, supernatant volume, enzyme inducer, and electron donor was investigated on the production process. The properties of synthesized nanoparticles were identified by using UV-Vis, FTIR, XRD, SEM, and EDS analysis.

Moreover, the growth curve of strain Ta-31 was plotted in the presence and absence of an enzyme inducer (concentration of 0.1 mM copper sulfate). After the phylogenetic analysis, 16S rDNA gene sequences were determined, and their phylogenetic tree of the selected strain was plotted. The results showed that the best conditions for producing CuNPs, glucose 1% as an electron donor, 2 mM copper sulfate, and 20 ml supernatant had the best production. Strain Ta-31 arrived at the end of the log phase and the beginning of the stationary phase after 15 h. CuNPs were spherical and irregular, and the size of CuNPs was more in the range of 30-40 nm. According to the results, strain Ta-31 belonged to Staphylococcus pasteuri sp. with 99.88% similarity.

Keywords

Staphylococcus pasteuri

Bioreactor

Copper nanoparticles

Green synthesis

20.1001.1.23222115.1401.13.4.9.8

Subjects

Nanotechnology

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