The interaction between MiR22 ,MiR194 with lncRNA MINCR in colorectal cancer tissiue

Cheraghi, Sajjad; Asadzadeh Aghdaie, Hamid

The interaction between MiR22 ,MiR194 with lncRNA MINCR in colorectal cancer tissiue

Document Type : Original Research

Authors

sajjad cheraghi ¹

hamid asadzadeh aghdaie ²

¹ islamic azad university tehran branch

² shahid beheshti university medical branch

Abstract

ntroduction: Colorectal cancer (CRC) is one of the most important cancers and the second leading cause of cancer mortality in Iran. CRC is spread through genetic and epigenetic changes. Understanding the molecular pathways in these genetic and epigenetic changes can provide a clear perspective on cancer treatment.
Materials and Methods: This study was performed on 40 samples of intestinal tumor growths (polyps), 30 tumor samples and 40 normal tissues adjacent to the tumor. RNA and tissue DNA extraction, study of gene expression, miR-22, miR-194 LncRNA MINCR using Real time PCR was performed.
Results: In this study, the expression of MINCR LncRNA in tumor and polyp samples increased compared to the adjacent normal tissue and the expression of miR-194 miR-22 was decreased. Negative correlation coefficient was observed between miR-22, miR-194 and MINCR expression levels and also a significant relationship was observed between the expression of these lncRNAs and microRNAs.

Keywords

lncRNA

microRNA

colorectal cancer miR22-miR194

20.1001.1.23222115.1401.14.1.3.9

Subjects

Molecular biotechnology

1-Dalmay T. MicroRNAs and cancer. Journal of internal medicine. 2008;263(4):366-75.
2-Garzon R, Calin GA, Croce CM. MicroRNAs in cancer. Annual review of medicine. 2009;60:167-79.
3-Borkman, R. F., Tassin , J. D., and Lerman, S. (1981) The rates of photodestruction Of tryptophan residues in human and bovine lens proteins. Exp. Eye Res. 32, 747-754
4-Wang S-H, Wu X-C, Zhang M-D, Weng M-Z, Zhou D, Quan Z-W. Long noncoding RNA H19 contributes to gallbladder cancer cell proliferation by modulated miR-194-5p targeting AKT2. Tumor Biology. 2016;37(7):9721-30.
5-Zhu X, Li D, Yu F, Jia C, Xie J, Ma Y, et al. miR-194 inhibits the proliferation, invasion, migration, and enhances the chemosensitivity of non-small cell lung cancer cells by targeting forkhead box A1 protein. Oncotarget. 2016;7(11):13139.
6-Wu X, Liu T, Fang O, Leach L, Hu X, Luo Z. miR-194 suppresses metastasis of non-small cell lung cancer through regulating expression of BMP1 and p27 kip1. Oncogene. 2014;33(12):1506.
7-Zhao H-J, Ren L-L, Wang Z-H, Sun T-T, Yu Y-N, Wang Y-C, et al. MiR-194 deregulation contributes to colorectal carcinogenesis via targeting AKT2 pathway. Theranostics. 2014;4(12):1193.
8-Wang S-H, Yang Y, Wu X-C, Zhang M-D, Weng M-Z, Zhou D, et al. Long non-coding RNA MINCR promotes gallbladder cancer progression through stimulating EZH2 expression. Cancer letters. 2016;380(1):122-
9-Zhang J, Yang Y, Yang T, Liu Y, Li A, Fu S, et al. microRNA-22, downregulated in hepatocellular carcinoma and correlated with prognosis, suppresses cell proliferation and tumourigenicity. British journal of cancer. 2010;103(8):1215.
10-Zhang J, Yang Y, Yang T, Liu Y, Li A, Fu S, et al. microRNA-22, downregulated in hepatocellular carcinoma and correlated with prognosis, suppresses cell proliferation and tumourigenicity. British journal of cancer. 2010;103(8):1215.
11-Thorpe LE, Mostashari F, Hajat A, Nash D, Karpati A, Weber T, et al. Colon cancer screening practices in New York City, 2003: results of a large random‐digit dialed telephone survey. Cancer: Interdisciplinary International Journal of the American Cancer Society. 2005;104(5):1075-82
12-Potter JD. Colorectal cancer: molecules and populations. Journal of the National Cancer Institute. 1999;91(11):916-32