(â1082G/A) Polymorphism in Interleukinâ10 Promoter and relation to Gastric Cancer by Tetra Arms-PCR

Alijabari, Shayesteh; Siasi, Elham; Rafiei Tabatabae, Robab

(â1082G/A) Polymorphism in Interleukinâ10 Promoter and relation to Gastric Cancer by Tetra Arms-PCR

Document Type : Original Research

Authors

shayesteh alijabari ¹

Elham Siasi ¹

Robab Rafiei Tabatabae ²

¹ Department of Genetic, School of science, Islamic Azad University, North Tehran Branch, Tehran, Iran.

² Department of Genetic, School of science, Islamic Azad University, North Tehran Branch, Tehran, Iran

Abstract

Aim: Gastric cancer is the second common cause of cancer death worldwide. Cytokines are mediators of peptides that are involved in the regulation of immunological responses, inflammatory systemic responses, and repair responses to risk factors. Interleukins are cytokines that are produced on other lymphocytes. Interleukin-10, is one of the body's most important interleukin that to inhibit inflammatory and immune responses. Different polymorphisms are found in the promoter region of interleukin 10 gene, which by changing the rate of this gene expression, could been altered its function. In this present research, was studied of relation between (-1082G/A) polymorphism in interleukin 10 gene and gastric cancer.

Material and methods: Two groups consist of 50 patients and 50 controls, were selected as the study samples population and were taken blood samples from them. Next DNA extraction from samples, genotyping of this polymorphism was used by Tetra-ARMS-PCR. Then was analyzed the results.

Results: Samples genotyping was showed frequency of A and G alleles 74% and 26%, in patient groups and in control groups 68% and 32%, respectively. There was a significant association between GG genotype in this polymorphism and gastric cancer (P=0.013).

Conclusion: This study results shown that can be used (-1082G/A) polymorphism in interleukin 10 gene as a molecular biomarker for gastric cancer in Iranian patients’ population.

Keywords

Gastric cancer

Interleukin 10 gene

Polymorphism

Tetra Arms PCR

Subjects

Molecular biotechnology

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