Investigation of bacterial diversity of Aq-Darreh gold mine and determination of their resistance to arsenic and silver

Moghadami, Fouzieh; Kavyanifard, Amirarsalan

Investigation of bacterial diversity of Aq-Darreh gold mine and determination of their resistance to arsenic and silver

Document Type : Original Research

Authors

Fouzieh Moghadami

Amirarsalan Kavyanifard

Department of Biology, Payame Noor University, P.B:19395-4697, Tehran, Iran.

Abstract

Microorganisms play an important role in formation of mines. In this research, the bacteria inhabiting in Aq-Darreh Takab gold mine were isolated and compared with agricultural soils. The isolates were characterized using 16S rDNA sequencing and the homology searches were performed using BlastN, EzTaxon, and RDP Classifier web tools. Resistance of the isolates was also investigated against arsenic and silver in the presence and absence of 3.5 ppm gold. Although the control soil showed a wide variety of bacterial diversity (43 isolates belonging to 13 genera), only 17 isolates belonging to 11 genera were isolated from mine soils including Acinetobacter, Agrobacterium, Comamonas, Deinococcus, Listeria, Microbacterium, Micrococcus, Pseudomonas, Rhizobium, Roseomonas and Staphylococcus. Among the isolates, A. radiobacter, D. ficus, M. antarcticus, M. luteus, R. radiobacter and R. selenitidurans were able to tolerate different amounts of arsenic and silver in the presence of gold, among which A. radiobacter and D. ficus showed the highest resistance in such a way that they grew in the presence of 50 ppm arsenic, 50 ppm silver, and 3.5 ppm gold. Our results showed that bacterial diversity in soils containing gold, silver and arsenic metals is less than agricultural soils. It was also found that the bacterial diversity in gold mines depends on the amount of gold and the amount and type of associated elements. Due to high resistance of two endogenous bacterial species to arsenic and silver, A. radiobacter and D. ficus, have also the potential for industrial purposes in environments contaminated with these metals.

Keywords

Aq-Darreh

Arsenic

Gold mine

Silver

Endogenous bacterium

Subjects

Industrial Biotechnology

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