Introduction of a biological safe method for establishing pathogen-free cultures in in vitro culture of iris (Iris hollandica cv. Apollo)

Taghizadeh, Mina

Introduction of a biological safe method for establishing pathogen-free cultures in in vitro culture of iris (Iris hollandica cv. Apollo)

Document Type : Original Research

Author

Mina Taghizadeh

Horticultural science, faculty of agricultural and natural resources, Arak University, Arak, Iran

Abstract

Pathogen growth in vitro is one of the major problems in plant micropropagation, so the most important stage of in vitro culture of plants is disinfection of the cultures. In the common methods of disinfection, the media and plant materials, apparatus such as autoclaves and chemicals disinfectents are used, which causes the time of this process and the costs to increase. This research objective to improve the disinfection of Iris hollandica cv. Apollo scales using different plant essential oils (thyme, cumin and savory) and the methods of using essential oils as disinfectants, the use of essential oils in the medium and the use of essential oils fumigation was done in four independent experiments. The use of essential oils of thyme, cumin, and savory completely prevented both contamination of the culture medium and contamination of the explant. The best disinfection method was when the essential oils were used in combination with the culture medium. Bacterial contamination was better controlled at concentrations of 0.125 to 0.25%, but the concentration of 0.25% of essential oils resulted in better control of fungal infection. The least browning of iris scale explants was observed at a concentration of 0.125. The technique presented in this study can significantly reduce the cost of electricity and lighting, as well as personnel costs. Therefore, this method can introduce a practical and cost-effective technique for plant micropropagation.

Keywords

Contamination

Cost-effective

Essential oil

Micropropagation

Sodium hypochlorite

Subjects

Agricultural Biotechnology

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