Effect of zinc oxide quantum dots on methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Staphylococcus aureus (VRSA) isolates from Baghdad Hospital by PCR-technique for mecA and vanA genes

Neamah Abbas, Zahraa; Naji Abdullah, Hanaa; Ranjbar, Bijan

Effect of zinc oxide quantum dots on methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Staphylococcus aureus (VRSA) isolates from Baghdad Hospital by PCR-technique for mecA and vanA genes

Document Type : Original Research

Authors

Zahraa Neamah Abbas ¹

Hanaa Naji Abdullah ²

Bijan Ranjbar ³

¹ Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, IranDepartment of Medical Techniques, Faculty of Health and Medical Techniques, Middle Technical University, Baghdad, Iraq

² Department of Medical Techniques, Faculty of Health and Medical Techniques, Middle Technical University, Baghdad, Iraq

³ Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran

Abstract

In this study, 50 Staphylococcus aureus samples from Baghdad Hospital were collected and examined, 17 samples were infected with methicillin-resistant Staphylococcus aureus (MRSA) and 5 samples were infected with vancomycin-resistant Staphylococcus aureus (VRSA). The sensitivity of the isolates against different antimicrobial agents was evaluated using the VITEK2 standard system. According to CLSI, the minimum inhibitory concentration (MIC) values of zinc oxide quantum dots (ZnO-QDs) were also tested by the Muller-Hinton dilution method. In addition, polymerase chain reaction (PCR) was performed to identify vanA and mecA genes. The antibacterial effects of ZnO-QDs on VRSA were higher than MRSA isolates.

Keywords

Staphylococcus aureus

mecA gene

vanA gene

Zinc oxide

Quantum dots

Subjects

Nanotechnology

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