Biosynthesis of Ag/AgCl nanocomposites based on bacterial strains Bacillus haynesii sp. PN14F and Bacillus halotolerans sp. B3, and investigation of their catalytic and antibacterial properties

Rakhshan, Narges; Mansournia, Mohammadreza; Jookar Kashi, Fereshteh

Biosynthesis of Ag/AgCl nanocomposites based on bacterial strains Bacillus haynesii sp. PN14F and Bacillus halotolerans sp. B3, and investigation of their catalytic and antibacterial properties

Document Type : Original Research

Authors

Narges Rakhshan ¹

Mohammadreza Mansournia ²

Fereshteh Jookar Kashi ¹

¹ University of Kashan

² university of Kashan

Abstract

The aim of present research is development of a simple green approach to produce Ag/AgCl nanocomposites using bacterial strains Bacillus haynesii and Bacillus halotorans, nominated at PN14F and B3, respectively, via an extracellular process. The bacterial strains PN14F and B3 were isolated from the soil and wastewater samples, using dilution and direct cultivation method. The Ag/AgCl nanocomposites were synthesized from the reaction of silver(I) nitrate solution and supernatant under completely sterile conditions in the presence of light. Moreover, a series of controlled experiments were provided to optimize some reaction conditions such as substrate concentration, PH, substrate volume, bacterial volume, the presence of glucose as an electron donor and silver(I) nitrate as inducer. The products were characterized using various techniques such as UV-Vis, XRD, FT-IR, FE-SEM and EDX. The resulting bionanocomposites (Ag1 and Ag2), with an average particle size of 30 and 22.3 nm, were efficient heterogeneous catalysts for reducing para-nitrophenol to para-aminophenl. Further, it was demonstrated their activity as the antibacterial properties against gram positive and negative bacteria. The results showed that the Ag2 sample with reducing time of 15 min is a more efficient catalyst than the Ag1 nanocomposite, which can be attributed to the smaller size of the Ag2 nanoparticles.

Keywords

Bionanocomposite

Ag/AgCl

Extracellular process

Heterogeneous catalyst

Antimicrobial activity

Subjects

Microbial biotechnology

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