study of A501R mutation role in  PFU DNA polymerase processivity improvement

Arab, Seyed Shahryar; Vafaee, Rayeheh

study of A501R mutation role in PFU DNA polymerase processivity improvement

Document Type : Original Research

Authors

Seyed Shahryar Arab

Rayeheh Vafaee

tarbiat modares university

Abstract

PFU DNA polymerse shows the lowest error rate in Polymerase Chain Reaction (PCR) but it seperates from DNA after about 20 nucleotides add to the end of primer strand. The research purpus is processivity improvement of PFU DNA polymerase by means of rational design and point mutation due to lowest enthropy and enthalpy costs. so DNA polymerases in B family with high processivity were selected and their structures and sequences were compared with PFU DNA polymerase then an optimized mutation was induced . Native form and mutant were simulated for 100 ns and the trajectories were analyzed. ΔG_bindingwas calculated by g_mmpbsa tool and proved that the mutant shows a robust affinity .

Keywords

processivity

DNA polymerase PFU

Molecular dynamics simulation

Subjects

Bioinformatics

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