Volume 9, Issue 4 (2018)                   JMBS 2018, 9(4): 495-500 | Back to browse issues page

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1- Horticulture Department, Agriculture Faculty, Guilan University, Guilan, Iran
2- Tissue & Cell Culture Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education & Extension Organization (AREEO), Karaj, Iran, Tissue & Cell Culture Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education & Extension Organization (AREEO), Karaj, Iran , m.j.kermani@abrii.ac.ir
Abstract:   (9080 Views)
Aims: The mass propagation and breeding new varieties of Ziziphus jujuba Mill as a valuable fruit tree and herb, which is well adapted to dry and semi-arid climate, is very important. The aim of the present research was to optimize direct regeneration method in Ziziphus jujuba Mill.
Materials and Methods: In this experimental study, the explants consisted of leaf cut into 3 parts, leaf cut from 4 sides, and full leaf of in vitro and compared in Murashige and Skoog and woody plant media with different concentrations of Thidiazuron (TDZ; 0, 5, 10, 15, and 20μM) and Naphthalene acetic acid (NAA, 0 and 1μM). The effect of 2 and 4 weeks of darkness on regeneration rate was investigated. The experiments were conducted in factorial based on a completely randomized design. The mean of statistical data was compared using Duncan’s multiple range test. SAS 9.3.1 software was used and the difference was considered significant at 1% probability level.
Findings: The 2 weeks of darkness treatment with the mean of 1.38 was better than the 4 weeks of darkness treatment. The Maximum number of shoots (2.27) was obtained in leaf cut into 3 parts. The maximum percent of regeneration (75.0%) and highest number of regenerated shoots (4.83) were obtained in the MS medium containing 10μΜ TDZ and 1μΜ NAA.
Conclusion: Regeneration rates in Ziziphus jujuba Mill is affected by the type of explant, culture media and plant growth regulators.   Maximum rate of regeneration is observed in leaf cut into 3 parts and cultured on MS medium containing 1μM NAA and 10μΜ TDZ. Plantlets are rooted and successfully acclimatized at “in vivo” conditions.
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Article Type: Research Paper | Subject: Agricultural Biotechnology
Received: 2016/07/23 | Accepted: 2018/02/21 | Published: 2018/12/21

References
1. Assareh MH, Sardabi H. Macropropagation and micropropagation of Ziziphus spina-christi. Pesquisa Agropecuária Brasileira. 2005;40(5):459-65. [Link] [DOI:10.1590/S0100-204X2005000500006]
2. Ghouth K. Ziziphus jujube the neglected fruit. Mashhad: Saeedi Manesh; 2009. [Persian] [Link]
3. Depommier D. Ziziphus mauritiana Lam, culture and use in Kapsiki (North Cameroon). Bois & Forêts des Tropiques existe depuis. 1988;218(4):57-62. [French] [Link]
4. Abass NH. Rapid in vitro Multiplication and ex vitro Rooting of Zizyphus mauritiana. J Appl Sci Res. 2010;6(1):63-9. [Link]
5. Chen ZL, Yan ZL, Xue H, Feng XD, Chen GL, Cao JY. In vitro culture of leaves and plantlet regeneration of Ziziphus jujube, Zhanhua Dongzao. Plant Physiol Commun. 2002;38(6):584. [Link]
6. Sudhersan C, Hussain J. In vitro clonal propagation of a multipurpose tree, Ziziphus spina-christi (L.) desf. Turk J Bot. 2003;27:167-71. [Link]
7. Al-Sulaiman MA, Barakat MN. In vitro shoot multiplication of Ziziphus spina-christi by shoot tip culture. Afr J Biotechnol. 2010;9(6):850-7. [Link] [DOI:10.5897/AJB09.1674]
8. Al-Sulaiman MA. Clonal propagation of Ziziphus spina-christi by shoot tip culture: I. improved inorganic and organic media constituents for in vitro shoot multiplication. J King Abdulaziz Univ, Meteorol, Environ Arid Land Agric Sci. 2010;21(2):3-17. [Link] [DOI:10.4197/met.21-2.1]
9. Feng JC, Yu XM, Shang XL, Li JD, Wu YX. Factors influencing efficiency of shoot regeneration in Ziziphus jujuba Mill. 'Huizao'. Plant Cell Tissue Organ Cult. 2010;101(1):111-7. [Link] [DOI:10.1007/s11240-009-9663-2]
10. Ma C, Ye X, Chen Y, Feng J, Shang X, Li J, et al. Anatomical observations of adventitious bud regeneration from leaf explants of Ziziphus jujube Mill. 'Huizao'. Hortic Environ Biotechnol. 2012;53(4):316-9. [Link] [DOI:10.1007/s13580-012-0081-8]
11. Pourhosseini L, Jafarkhani Kermani M, Habashi AA, Khalighi A. Effciency of direct and indirect shoot organogenesis in different genotypes of Rosa hybrida. Plant Cell Tissue Organ Cult. 2013;112(1):101-8. [Link] [DOI:10.1007/s11240-012-0210-1]
12. Dohm A, Ludwig C, Schilling D, Debener T. Transformation of roses with genes for antifungal proteins to reduce their susceptibility to fungal diseases, Int Soc Hortic Sci, Acta Hortic. 2002;572:105-11. [Link] [DOI:10.17660/ActaHortic.2002.572.12]
13. Zakizadeh H, Stummann BM, Lütken H, Müller R. Isolation and characterization of four somatic embryogenesis receptor-like kinase (RhSERK) genes from miniature potted rose (Rosa hybrida cv. Linda). Plant Cell Tissue Organ Cult. 2010;101(3):331-8. [Link] [DOI:10.1007/s11240-010-9693-9]
14. Kucharska D, Orlikowska T. Enhancement of in vitro organogentic capacity of rose by preculture of donor shoots on the medium with thidiazuron. Acta Physiol Plant. 2009;31(3):495-500. [Link] [DOI:10.1007/s11738-008-0258-z]
15. Fasolo F, Zimmerman RH, Fordham I. Adventitious shoot formation on excised leaves of in vitro grown shoots of apple cultivars. Plant Cell Tissue Organ Cult. 1989;16(2):75-87. [Link] [DOI:10.1007/BF00036516]
16. Chevreau E, Skirvin R, Abu-Qaoud HA, Korban SS, Sullivan JG. Adventitious shoot regeneration from leaf tissue of three pear (Pyrus sp.) cultivars in vitro. Plant Cell Rep. 1989;7(8):688-91. [Link]
17. Van Nieuwkerk JP. Thidiazuron stimulation of apple shoot proliferation in vitro. Hortic Sci. 1986;21(3):516-18. [Link]
18. Yusnita S, Geneve RL, Kester ST. Micropropagation of white flowering eastern redbud (Cercis canadensis var. alba L.). J Environ Hortic. 1990;8(4):177-9. [Link]
19. Gu XF, Zhang JR. An efficient adventitious shoot regeneration system for Zhanhua winter jujube (Zizyphus jujuba Mill.) using leaf explants. Plant Cell Rep. 2005;23(12):775-9. [Link] [DOI:10.1007/s00299-005-0920-5]
20. Ahn YJ, Vang L, McKeon TA, Chen GQ. High-frequency plant regeneration through adventitious shoot formation in castor (Ricinus communis L.). Vitro Cell Dev Biol Plant. 2007;43(1):9-15. [Link] [DOI:10.1007/s11627-006-9009-2]
21. Beck MJ, Camper ND. Shoot regeneration from petunia leaf discs as a function of explants size, configuration and benzyladenine exposure. Plant Cell Tissue Organ Cult. 1991;26(2):101-6. [Link] [DOI:10.1007/BF00036113]

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