Volume 9, Issue 3 (2018)                   JMBS 2018, 9(3): 459-464 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Haghighat N, Abdolmaleki P, Behmanesh M, Parnian J. Effect of Electromagnetic Field and Nitric Oxide on the Neural Differentiation Proteins Marker and Viability of the Rat Bone Marrow Mesenchymal Stem Cells. JMBS 2018; 9 (3) :459-464
URL: http://biot.modares.ac.ir/article-22-14104-en.html
1- Biophysics Department, Biology Science Faculty, Tarbiat Modares University, Tehran, Iran
2- Biophysics Department, Biology Science Faculty, Tarbiat Modares University, Tehran, Iran, Biophysics Department, Biology Science Faculty, Tarbiat Modares University, Nasr Bridge, Jalal-Al-Ahmad Highway, Tehran, Iran , Parviz@modares.ac.ir
3- Genetics Department, Biology Science Faculty, Tarbiat Modares University, Tehran, Iran
4- Biotechnology Institute, Iranian Research Organization For Science & Technology, Tehran, Iran
Abstract:   (3938 Views)
Aims: Nitric oxide (NO) plays an important role in maintaining cellular stem status, and the range of electromagnetic fields (EMF) is very deep in contrast to the electric field. The aim of this study was to investigate the effect of electromagnetic field and nitric oxide on the neural differentiation proteins marker and viability of the rat bone marrow mesenchymal stem cells.
Materials and Methods: The present experimental research was conducted on bone marrow mesenchymal stem cells of Vistar rats. For treatments of the cells, high (1mM) and low (10micromolar Deta-NO) concentrations were used as a nitric oxide donor molecule and 50Hz low-frequency electromagnetic field and they were compare with the control group. The cell viability was recorded by MTT assay test, the neural differentiation pathway gene expression was investigated by RT-PCR technique, and the neural differentiation marker protein expression was evaluated by Immunocytochemistry technique. The data were analyzed by one-way ANOVA, using SPSS 13 software.
Findings: After 24 hours of treatment with nitric oxide and EMF, the rate of viability in all groups was significantly decreased compared to the control group. After 48 hours, EMF alone, as well as with low concentration of nitric oxide did not decrease the rate of viability and cell growth increased compared to the control group. In the group treated with high nitric oxide concentration along with EMF, MAP2 protein was expressed in the number of cells more than the control group and the one treated with EMF.
Conclusion: The electromagnetic field, along with its high concentration of nitric oxide, decreases the number of rat bone marrow mesenchymal stem cells and, by increasing cell size, gene expression and neural differentiation proteins marker facilitates their differentiation to nerve-like cells.
Full-Text [PDF 840 kb]   (2683 Downloads)    
Article Type: Research Paper | Subject: Agricultural Biotechnology
Received: 2016/09/26 | Accepted: 2017/09/3 | Published: 2018/09/22

1. Eichholz GG. Non-ionizing radiation, part 1: Static and Extremely Low-Frequency (ELF) electric and magnetic fields. Health Phys. 2002;83(6):920. [Link] [DOI:10.1097/00004032-200212000-00021]
2. Ross CL, Siriwardane M, Almeida-Porada G, Porada CD, Brink P, Christ GJ, et al. The effect of low-frequency electromagnetic field on human bone marrow stem/progenitor cell differentiation. Stem Cell Res. 2015;15(1):96-108. [Link] [DOI:10.1016/j.scr.2015.04.009]
3. Ascenzi P, Di Masi A, Sciorati C, Clementi E. Peroxynitrite - an ugly biofactor?. Biofactors. 2010;36(4):264-73. [Link] [DOI:10.1002/biof.103]
4. Beltran-Povea A, Caballano-Infantes E, Salguero-Aranda C, Martín F, Soria B, Bedoya FJ, et al. Role of nitric oxide in the maintenance of pluripotency and regulation of the hypoxia response in stem cells. World J Stem Cells. 2015;7(3):605-17. [Link] [DOI:10.4252/wjsc.v7.i3.605]
5. Bonafè F, Guarnieri C, Muscari C. Nitric oxide regulates multiple functions and fate of adult progenitor and stem cells. J Physiol Biochem. 2015;71(1):141-53. [Link] [DOI:10.1007/s13105-014-0373-9]
6. Chuang JH, Tung LC, Lin Y. Neural differentiation from embryonic stem cells in vitro: An overview of the signaling pathways. World J Stem Cells. 2015;7(2):437-47. [Link] [DOI:10.4252/wjsc.v7.i2.437]
7. Pilla AA. Electromagnetic fields instantaneously modulate nitric oxide signaling in challenged biological systems. Biochem Biophys Res Commun. 2012;426(3):330-3. [Link] [DOI:10.1016/j.bbrc.2012.08.078]
8. Förstermann U, Li H. Therapeutic effect of enhancing endothelial Nitric Oxide Synthase (eNOS) expression and preventing eNOS uncoupling. Br J Pharmacol. 2011;164(2):213-23. [Link] [DOI:10.1111/j.1476-5381.2010.01196.x]
9. Liu L, Wang D, Wang J, Ji H, Zhang Y. NOAD, a novel nitric oxide donor, induces G2/M phase arrest and apoptosis in human hepatocellular carcinoma Bel-7402 cells. Toxicol In Vitro. 2015;29(7):1289-97. [Link] [DOI:10.1016/j.tiv.2015.05.003]
10. Tejedo JR, Tapia-Limonchi R, Mora-Castilla S, Cahuana GM, Hmadcha A, Martin F, et al. Low concentrations of nitric oxide delay the differentiation of embryonic stem cells and promote their survival. Cell Death Dis. 2010;1:e80. [Link] [DOI:10.1038/cddis.2010.57]
11. Park JE, Seo YK, Yoon HH, Kim CW, Park JK, Jeon S. Electromagnetic fields induce neural differentiation of human bone marrow derived mesenchymal stem cells via ROS mediated EGFR activation. Neurochem Int. 2013;62(4):418-24. [Link] [DOI:10.1016/j.neuint.2013.02.002]
12. Choi BM, Pae HO, Jang SI, Kim YM, Chung HT. Nitric oxide as a pro-apoptotic as well as anti-apoptotic modulator. J Biochem Mol Biol. 2002;35(1):116-26. [Link]
13. Huang NF, Fleissner F, Sun J, Cooke JP. Role of nitric oxide signaling in endothelial differentiation of embryonic stem cells. Stem Cells Dev. 2010;19(10):1617-26. [Link] [DOI:10.1089/scd.2009.0417]
14. Nott A, Riccio A. Nitric oxide-mediated epigenetic mechanisms in developing neurons. Cell Cycle. 2009;8(5):725-30. [Link] [DOI:10.4161/cc.8.5.7805]
15. Charles N, Ozawa T, Squatrito M, Bleau AM, Brennan CW, Hambardzumyan D, et al. Perivascular nitric oxide activates notch signaling and promotes stem-like character in PDGF-induced glioma cells. Cell Stem Cell. 2010;6(2):141-52. [Link] [DOI:10.1016/j.stem.2010.01.001]
16. Pacher P, Beckman JS, Liaudet L. Nitric oxide and peroxynitrite in health and disease. Physiol Rev. 2007;87(1):315-424. [Link] [DOI:10.1152/physrev.00029.2006]

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.