Volume 10, Issue 3 (2019)                   JMBS 2019, 10(3): 373-380 | Back to browse issues page

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1- Micribial Biotechnology Department, Basic Sciences Faculty, Payame Noor University, Tehran, Iran
2- Nanobiotechnology Department, Biological Sciences Faculty, Tarbiat Modares University, Tehran, Iran, Tarbiat Modares University, Nasr Bridge, Jalal-Al-Ahmad Highway, Tehran, Iran. , tamjid@modares.ac.ir
3- Biochemistry Department, Basic Sciences Faculty, Payame Noor University, Tehran, Iran
Abstract:   (6804 Views)

Aims: Recently, polymer-based nanofibrous scaffolds have attracted great attention due to their significant antibacterial properties in the field of dermatological applications. In this study, a polycaprolactone-based nanofibrous scaffold has been fabricated using the electrospinning method. The aim of this study was to evaluate the antibacterial effect of electrospun nanofibrous structures. Materials and Methods: In this experimental study, the structure and bacterial attachment on polymeric nanofibrous scaffolds were studied by Scanning Electron Microscopy (SEM). In addition, antibacterial properties of nanofibrous scaffolds were studied on two gram-negative bacteria of Escherichia coli and Pseudomonas aeruginosa and two gram-positive bacteria of Staphylococcus aureus and Streptococcus mutans, using microdilution method and biofilm assay. Moreover, MTT assay was performed on HeLa and human fibrosarcoma cell line (HT1080) cancerous cell lines to evaluate the cell viability.
Findings: The results of this study showed that nanofibrous scaffold revealed a significant antimicrobial and anti-biofilm formation effect on all of the studied bacterial strains, but in microscopic observations and microdilution assay was observed on Pseudomonas aeruginosa in 1mg/ml of nanofibrous scaffold extract concentration, while the major effect in biofilm assay was observed in 8µg/ml of extract concentration. Moreover, the cell viability studies showed that the most significant effect was shown on HT1080 cell line which has drastically decreased by 40% after 48 hours in comparison with the control.
Conclusion: These results show that electrospun nanofibrous PCL-based scaffolds are potentially promising for dermal tissue engineering applications, due to anti-biofilm effects and capability of reducing the number of cancerous cells in the wound site.

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Article Type: Original Research | Subject: Pharmaceutical Biotechnology
Received: 2018/08/1 | Accepted: 2018/07/5 | Published: 2019/09/21

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