Volume 11, Issue 1 (2020)
JMBS 2020, 11(1): 61-69 |
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Bohlouli M, Tamjid E, Mohammadi S, Nikkhah M. A study on cytotoxicity, hemocompatibility, and antibacterial properties of tetracycline hydrochloride-loaded PCL-based composite scaffolds for bone tissue engineering. JMBS 2020; 11 (1) :61-69
URL: http://biot.modares.ac.ir/article-22-28528-en.html
URL: http://biot.modares.ac.ir/article-22-28528-en.html
1- Department of Interdisciplinary Science and Technology, Tarbiat Modares University
2- Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tarbiat Modares University, Tehran, P.O. Box 14115-175, Iran ,tamjid@modares.ac.ir
3- Department of Nanobiotechnology, Tarbiat Modares UniversityCurrent affiliation: Nano Drug Delivery Research Center, Kermanshah University of Medical Sciences
4- Department of Nanobiotechnology, Tarbiat Modares University
2- Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tarbiat Modares University, Tehran, P.O. Box 14115-175, Iran ,
3- Department of Nanobiotechnology, Tarbiat Modares UniversityCurrent affiliation: Nano Drug Delivery Research Center, Kermanshah University of Medical Sciences
4- Department of Nanobiotechnology, Tarbiat Modares University
Abstract: (4067 Views)
Since one of the main problems in bone tissue repair is the bacterial infections, recently the development of drug-eluting nanocomposite scaffolds for bone regenerative medicine applications has attracted significant attention. In this study Polycaprolactone (PCL)-based composite scaffolds containing 10vol% of titanium dioxide nanoparticles (~21nm), and bioactive glass particles (~6µm), were prepared without drug and also loaded by Tetracycline hydrochloride (TCH) antibiotic (0.57, 1.15 mg/mL) through solvent casting method for bone tissue engineering applications. Structural characterizations based on Scanning Electron Microscopy (SEM), and FTIR analysis were utilized to study the chemical bonds of glass/ceramic particles, and antibiotic crystals on the surface. In addition, in vitro cytotoxicity, and antibacterial analysis were performed by MTT, and Agar well-diffusion assays, respectively. In this study polymeric and composite scaffolds were fabricated with TCH clusters decorated on the surface. It was shown that the bioactive glass/PCL scaffolds loaded by 0.57 mg/mL of TCH revealed significant antibacterial effect, despite the acceptable cell viability. These scaffolds seem to be of interest as a potential candidate in drug-eluting scaffolds for bone tissue engineering applications.
Keywords: Bone tissue engineering, Solvent casting, Tetracycline hydrochloride, Cytotoxicity, Antibacterial effect, composite scaffold
Article Type: Original Research |
Subject:
Pharmaceutical Biotechnology
Received: 2018/12/24 | Accepted: 2019/10/13 | Published: 2020/06/6
Received: 2018/12/24 | Accepted: 2019/10/13 | Published: 2020/06/6
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