Volume 10, Issue 4 (2019)
JMBS 2019, 10(4): 573-580 |
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Hashemzadeh H, Allahverdi A, Ertl P, Naderi-Manesh H. Comparison between Three-Dimensional Spheroid and Two-Dimensional Monolayer in A549 Lung Cancer and PC9 Normal Cell Lines under Treatment of Silver Nanoparticles. JMBS 2019; 10 (4) :573-580
URL: http://biot.modares.ac.ir/article-22-29683-en.html
URL: http://biot.modares.ac.ir/article-22-29683-en.html
1- Nanobiotechnology Department, Biosciences Faculty, Tarbiat Modares University, Tehran, Iran
2- Biophysics Department, Biosciences Faculty, Tarbiat Modares University, Tehran, Iran
3- Institute of Applied Synthetic Chemistry, Vienna University of Technology, Vienna, Austria
4- Nanobiotechnology Department, Biosciences Faculty, Tarbiat Modares University, Tehran, Iran, Tarbiat Modares University, Nasr Bridge, Jalal-Al-Ahmad Highway, Tehran, Iran. Postal Code: 1411713116 ,naderman@modares.ac.ir
2- Biophysics Department, Biosciences Faculty, Tarbiat Modares University, Tehran, Iran
3- Institute of Applied Synthetic Chemistry, Vienna University of Technology, Vienna, Austria
4- Nanobiotechnology Department, Biosciences Faculty, Tarbiat Modares University, Tehran, Iran, Tarbiat Modares University, Nasr Bridge, Jalal-Al-Ahmad Highway, Tehran, Iran. Postal Code: 1411713116 ,
Abstract: (6132 Views)
In view of the constant increase of nanotechnology and nanomaterials applications in our daily life, to determine whether they are safe, “in vitro” and “in vivo” screening methods are needed. Obviously, application of models that are similar to the physiological tissues process of the human body could be a better candidate. The three-dimensional spheroid method, spheroid were generated using commercial microplates, has many benefits (in comparison with traditional methods or monolayer cell culture) such as the growth of the cells in 3D, similar to the body's physiological tissue, an alternative for animal models, cell-to-cell interactions, and better cell signaling. In this study, the toxicity of silver nanoparticles by using three factors such as metabolic activity, live/dead assay, and spheroid surface area was evaluated using two different methods (2D vs 3D) under treatment with various concentrations of silver nanoparticles at different times. The results showed that different cells types, cancer and/or normal lung cells, have significant differences. In addition, it was observed that distinct differences in terms of cytotoxicity of silver nanoparticles between 2D and 3D culture systems and also the rate of growth/non-growth of spheroids are highly depended on cell type and various concentrations have fundamental importance in such studies. The present study provides evidence that cellular dimensions (3D vs 2D) play a pivotal role in the results and outcomes of inflammation and cytotoxicity with nanoparticles due to the spatial-temporal structure.
Article Type: Original Research |
Subject:
Nanotechnology
Received: 2019/01/23 | Accepted: 2019/02/27 | Published: 2019/12/21
Received: 2019/01/23 | Accepted: 2019/02/27 | Published: 2019/12/21
References
1. Ulusoy M, Lavrentieva A, Walter JG, Sambale F, Green M, Stahl F, et al. Evaluation of CdTe/CdS/ZnS core/shell/shell quantum dot toxicity on three-dimensional spheroid cultures. Toxicol Res. 2016;5(1):126-35. [Link] [DOI:10.1039/C5TX00236B]
2. Chatzinikolaidou M. Cell spheroids: The new frontiers in in vitro models for cancer drug validation. Drug Discov Today. 2016;21(9):1553-60. [Link] [DOI:10.1016/j.drudis.2016.06.024]
3. Pampaloni F, Reynaud EG, Stelzer EH. The third dimension bridges the gap between cell culture and live tissue. Nat Rev Mol Cell Biol. 2007;8(10):839-45. [Link] [DOI:10.1038/nrm2236]
4. Eilenberger Ch, Kratz SRA, Rothbauer M, Ehmoser EK, Ertl P, Küpcü S. Optimized alamarBlue assay protocol for drug dose-response determination of 3D tumor spheroids. MethodsX. 2018;5:781-7. [Link] [DOI:10.1016/j.mex.2018.07.011]
5. Hoffmann OI, Ilmberger C, Magosch S, Joka M, Jauch KW, Mayer B. Impact of the spheroid model complexity on drug response. J Biotechnol. 2015;205:14-23. [Link] [DOI:10.1016/j.jbiotec.2015.02.029]
6. Darvishi MH, Nomani A, Hashemzadeh H, Amini M, Shokrgozar MA, Dinarvand R. Targeted DNA delivery to cancer cells using a biotinylated chitosan carrier. Biotechnol Appl Biochem. 2017;64(3):423-32. [Link] [DOI:10.1002/bab.1497]
7. Zabarjadi AR, Hashemzadeh H, Taravat E. Telomere, chromosome end and telomerase enzyme as a cancer biomarker. Genet Third Millenn. 2013;11(1):3019-27. [Persian] [Link]
8. Rashmezad MA, Ali Asgary E, Tafvizi F, Sadat Shandiz SA, Ataollah S, Mirzaie A. Comparative study on cytotoxicity effect of biological and commercial synthesized nanosilver on human gastric carcinoma and normal lung fibroblast cell lines. Tehran Univ Med J. 2015;72(12):799-807. [Persian] [Link]
9. Stensberg MC, Wei Q, McLamore ES, Porterfield DM, Wei A, Sepúlveda MS. Toxicological studies on silver nanoparticles: Challenges and opportunities in assessment, monitoring and imaging. Nanomedicine. 2011;6(5):879-98. [Link] [DOI:10.2217/nnm.11.78]
10. Akter M, Sikder MT, Rahman MM, Ullah AKMA, Hossain KFB, Banik S, et al. A systematic review on silver nanoparticles-induced cytotoxicity: Physicochemical properties and perspectives. J Adv Res. 2018;9:1-16. [Link] [DOI:10.1016/j.jare.2017.10.008]
11. Jaworski S, Wierzbicki M, Sawosz E, Jung A, Gielerak G, Biernat J, et al. Graphene oxide-based nanocomposites decorated with silver nanoparticles as an antibacterial agent. Nanoscale Res Lett. 2018;13(1):116. [Link] [DOI:10.1186/s11671-018-2533-2]
12. Monnappa AK, Bari W, Choi SY, Mitchell RJ. Investigating the responses of human epithelial cells to predatory bacteria. Sci Rep. 2016;6:33485. [Link] [DOI:10.1038/srep33485]
13. Selvi BCG, Madhavan J, Santhanam A. Cytotoxic effect of silver nanoparticles synthesized from Padina tetrastromatica on breast cancer cell line. Adv Nat Sci Nanosci Nanotechnol. 2016;7(3):035015. [Link] [DOI:10.1088/2043-6262/7/3/035015]
14. Nallathamby PD, Xu XH. Study of cytotoxic and therapeutic effects of stable and purified silver nanoparticles on tumor cells. Nanoscale. 2010;2(6):942-52. [Link] [DOI:10.1039/c0nr00080a]
15. Walzl A, Unger Ch, Kramer N, Unterleuthner D, Scherzer M, Hengstschläger M, et al. The resazurin reduction assay can distinguish cytotoxic from cytostatic compounds in spheroid screening assays. J Biomol Screen. 2014;19(7):1047-59. [Link] [DOI:10.1177/1087057114532352]
16. Chia SL, Tay CY, Setyawati MI, Leong DT. Biomimicry 3D gastrointestinal spheroid platform for the assessment of toxicity and inflammatory effects of zinc oxide nanoparticles. Small. 2015;11(6):702-12. [Link] [DOI:10.1002/smll.201401915]
17. Zanoni M, Piccinini F, Arienti Ch, Zamagni A, Santi S, Polico R, et al. 3D tumor spheroid models for in vitro therapeutic screening: A systematic approach to enhance the biological relevance of data obtained. Sci Rep. 2016;6:19103. [Link] [DOI:10.1038/srep19103]
18. Chu LH, Chen BS. Comparisons of robustness and sensitivity between cancer and normal cells by microarray data. Cancer Inform. 2008;6:165-81. [Link] [DOI:10.4137/CIN.S386]
19. Senyavina NV, Gerasimenko TN, Pulkova NV, Maltseva DV. Transport and toxicity of silver nanoparticles in HepaRG cell spheroids. Bull Exp Biol Med. 2016;160(6):831-4. [Link] [DOI:10.1007/s10517-016-3321-6]
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