Volume 12, Issue 2 (2022)                   JMBS 2022, 12(2): 128-139 | Back to browse issues page

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Mehdizadeh M, sheykhpour M, Salahshourifar I, Siadat S D, Saffarian P. Investigating the effect of functionalized carbon nanotube nanofluid on klebsiella pneumoniae. JMBS 2022; 12 (2) :128-139
URL: http://biot.modares.ac.ir/article-22-50558-en.html
Investigating the effect of functionalized carbon nanotube nanofluid on klebsiella pneumoniae
Maryam Mehdizadeh1 , Mojgan Sheykhpour * 2, Iman Salahshourifar3 , Seyed Davar Siadat4 , Parvaneh Saffarian3
1- Department of Biology, School of basic sciences, science and research branch, Islamic Azad University, Tehran, Iran, Number 24. Alley 9, Taavon Bulvard, Ammar Yaser, Qom, Iran
2- Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran, Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran , m-sheikhpour@pasteur.ac.ir
3- Department of Biology, School of basic sciences, science and research branch, Islamic Azad University, Tehran, Iran
4- Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran
Abstract:   (2477 Views)
Klebsiella pneumoniae is a gram-negative bacillus of the Enterobacteriaceae family. Despite being part of the natural human microflora, this is an opportunistic pathogen and a major cause of nosocomial infections. The increased emergence of multidrug resistance in Klebsiella pneumoniae has limited the treatment options for this bacterium. Carbon nanotubes (CNT), by improving the stability and solubulity of drugs, could increase the effectiveness of drugs for treatment. The aim of this study is to investigate the antibacterial effect of nanofluid containing functionalized multi-walled carbon nanotubes (f-CNT-NF) on Klebsiella pneumoniae isolated from clinical specimens. For the strain confirmation, biochemical ,API20E kit, and additional differential tests were performed, and antibiotic susceptibility test was performed by the disk diffusion method. The studied strain showed a resistance to all antibiotics such as cefepime.The minimum inhibitory concentration (MIC) was determined using the antibiotic micro dilution method. The MIC was determined in five effect modes including antibiotic (Ab), nanofluid containing functionalized multi-walled carbon nanotubes (f-CNT-NF) , nanofluid containing multi-walled carbon nanotubes (CNT-NF) ,Ab in combination with f-CNT-NF and Ab with CNT-NF. Nevertheless the individual effects of 10 µg mL-1 cefepime or 80 µg of nanofluid with f-CNT-NF did not inhibit the growth of the bacteria, but the co-administration of 10 µg mL-1 cefepime with 80 µg of the f-CNT-NF could inhibit the bacteria`s growth. It was concluded that f-CNT-NF could be more effective in drug delivery at lower concentrations than the free state, which could be used as a tool for optimal drug delivery.
Keywords: klebsiella pneumoniae, nanofluid, CNT, drug delivery, cefepime, MIC, Minimum Inhibitory concentration, Multi-walled carbon nanotube, Functionalized carbon nanotube
Full-Text [PDF 1789 kb]   (1171 Downloads)    
Article Type: Original Research | Subject: Microbial biotechnology
Received: 2021/02/28 | Accepted: 2021/04/18 | Published: 2022/01/30
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