Volume 5, Issue 2 (2014)                   JMBS 2014, 5(2): 29-44 | Back to browse issues page

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Investigation, identification and heritability of S and F alleles and vegetative characteristics in almond hybrids using morphological and molecular (PCR) markers. JMBS 2014; 5 (2) :29-44
URL: http://biot.modares.ac.ir/article-22-5186-en.html
Abstract:   (10607 Views)
Self-incompatibility is one of the most important difficulties in almond production which dramatically reduces fruit set and makes orchard management difficult. Therefore, breeding almond to produce self-compatible genotypes is very important. In this study, identification and screening of 100 almond hybrids obtained from cross between Touno (male parent) and A200 (female parent) after the self pollination by PCR reaction with specific primers of SfF and SfR. PCR results confirmed the situation of self-compatible hybrids. In addition, it indicated that, frequencies of Sf, S1 and S9 were 64%, 54% and 72% respectively, among hybrids. Hybrids and their parents vegetative traits (tree height, crown diameter, canopy diameter, branch number, the highest branch, leaf fall, lad length and across petiole length and glad number) using randomized complete bloke design (RCBD) in triplicate was done. PCR results confirmed the situation of self-compatible hybrids. In addition, it indicated that, 50% of genotypes were realized as self-compatible and another 50% were self-incompatible. Also primary evaluating of morphological traits of hybrids and their parents showed that most measured characters of hybrids was ranked between parents at present research, identified Self-compatible hybrids had been identified that can be used in almond breeding programs particularly to development the monoculture orchards of almond.
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Article Type: _ | Subject: Biotechnology
Received: 2013/06/11 | Accepted: 2015/02/20 | Published: 2015/05/24

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