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Synthesis of polymersome nanoparticles as carriers of podophyllotoxin bioactive compound with a proposed application against liver cancer
Parvaneh Peyvand * 1, Zahra Vaezi2 , Hossein Naderi-Manesh2
1- Tarbiat Modares University , payvand.p70@gmail.com
2- Tarbiat Modares University
Abstract:   (21 Views)
Hepatocellular carcinoma, predominant form of liver cancer, is the main cause of death in patients with liver cirrhosis. Podophyllotoxin, a natural anticancer compound, has ideal anti-tumor properties. However, its use is limited due to poor solubility and bioavailability. Finding a suitable drug delivery system have great importance in improving the bioavailability of podophyllotoxin. In this study, mPEG-PCL nanoparticles have been used for delivery of podophyllotoxin to liver cancer cells. mPEG-PCL copolymers were synthesized and characterized by DLS, FTIR and NMR analyses methods. The critical micellization concentration was 0.055 µg/ml. The z-average and surface charge of micelle was 186 ± 12 nm and -5.13 mV, respectively. podophyllotoxin was loaded in micelles in different w/w ratios of drug: copolymer. The size of the nanodrug was 214 ± 20 nm and the weight ratio of 1:1 with encapsulation efficiency of 77.36 ± 1.23 % was selected as the optimal ratio. The drug release results showed a significant difference between the rapid release of free podophyllotoxin and the more stable release of the loaded drug. At 37°C, drug release was higher, which was attributed to the destruction of polymersome structure at this temperature. According to the cytotoxicity study, the IC50 value for nanodrug (8.64 μg/ml) was lower than the IC50 value for the free drug (12.79 μg/ml), which showed the effect of improved cytotoxicity of nanodrug compared to the free drug. The results proved the polymersome can be potential carriers for delivery, controllable release and improve the toxicity effect of podophyllotoxin in cancer chemotherapy.
 
Keywords: Liver cancer, Podophyllotoxin, Polymersome, drug delivery, mPEG-PCL copolymer
     
Article Type: Original Research | Subject: Nanotechnology
Received: 2024/06/13 | Accepted: 2024/09/8
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