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In this study,we investigated the biosurfactant -producing microorganisms . Samples of the oil wells , , stable and Ilam were collected and transferred to the Scientific and Industrial Research Organisation biotechnology collaboration of 45 strains isolated from the IROST and the best combinations of isolates selected strains produced using different culture conditions were the order of 3M mineral salts medium containing glucose , molasses and Lindhard Taguchi method was used in these experiments, various factors were analyzed . The best conditions are obtained to produce rhamnolipid with medium Lindhard in vitro rate of 8/8 g rhamnolipid and ability emulsification of crude oil, 88 percent of the dry weight of cell 2/2 g , therefore, the intended environment for the production the fermenter was used in the state parameters shaking, C/N ratio and aeration efficiency and maximum production of rhamnolipid 2/14 grams per liter of capacity emulsification of crude oil 2/98 and the dry cell 4/3 g of was 23 % and the highest oil recovery of crude oil saturation in the core when the system is equal to 42% was achieved.

Keywords: Biosurfactant, Psuedomonas aeruginosa, Rhamnolipid, MEOR, Isolated Oil Well Microorganisem

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