Aims: Bordetella pertussis is a gram negative and obligated aerobic bacterium that causes pertussis disease and it is a specific pathogen in human. Pertussis is an acute respiratory infection and leads to death in infants. The aim of this study was to analyze housekeeping genes in Bordetella pertussis vaccine strains by multilocus sequence typing (MLST).
Materials and Methods: In the present experimental study, 4 samples of 134 and 509 bacterial strains and 2 standard samples of Tohama I, and 18323 were collected. After biochemical tests, the samples were cultured and separated and the genomic purification of DNA was done by Phenol–chloroform technique and analyzed by MLST. After genome sequencing, the analysis was performed by standard software such as Clustalw 2, MEGA 5.04, and DNASIS Max 3. Sequence similarity of 16S rDNA gene nucleotides was performed, using BLAST software with sequences recorded in the GenBank genome database to compare and determine the sequences similarity.
Findings: Regarding the created bands and the sequence of the game, the housekeeping genes in Bordetella pertussis vaccine strains were approved. The results of the PCR reaction for Pgm, Icd, Gly A, and Tyr B genes showed that all specimens have homogenous genes with a molecular weight of 500bp.
Conclusion: Evaluating the housekeeping genes in Bordetella pertussis vaccine strains by MLST vaccine strains (Razi Institute; Iran) correspond with international standard series and no change or deviation has occurred in the studied genes.

Aims: Migraine is a common, painful and, in severe and chronic cases, debilitating disease characterized by frequent, unilateral and pulsating attacks with moderate to severe intensity, sensitivity to light, nausea or vomiting. The aim of this study was to investigate the effectiveness of Transcranial Direct Current Stimulation [tDCS] on headache sign and sleep quality in migraine patients.
Method and Materials: The current research design was a semi-experimental type of pre- test, post-test with 2-week follow-up. The statistical population of this study was all patients suffering from migraine in Yazd city, a sample of 30 people was selected using the available sampling method and they were placed in two experimental and control groups by random sampling. The tools used in this research were Ahvaz Migraine Questionnaire and Pittsburgh Sleep Quality Questionnaire. The tDCS treatment method included 5 sessions of 20 minutes with a current of 2 mA .The data were analyzed using the analysis of covariance method.
Findings: The results showed that there is a significant difference between the experimental and control groups in the scores of headache sign and sleep quality in the post-test and follow-up [p<0/01].
Conclusion: The study showed that tDCS treatment has an effective role in improving headache sign and sleep quality.