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Total-Internal-Reflection Fluorescence Microscopy (TIRFM) is a useful tool to visualize and record the phenomena that happens below 100 nm thickness of the sample surface. This unique property of TIRFM help to perform a "qualitative" study of cytoskeleton near the cell-substrate contact. Here,   distribution of actin filaments at cell-substrate interface was imaged by a TIRFM set up. Then, staining the actins cytoskeleton of the human melanoma cell and implementing the prism-based total-internal-reflection fluorescence microscope.  A method to "quantify" distribution of fluorophores at cell-substrate contact is proposed.