Showing 4 results for Hamidoghli
Z. Hashemzadeh , M. Jafarkhani Kermani , Y. Hamidoghli ,
Volume 9, Issue 4 (Fall 2018)
Abstract
Aims: The mass propagation and breeding new varieties of Ziziphus jujuba Mill as a valuable fruit tree and herb, which is well adapted to dry and semi-arid climate, is very important. The aim of the present research was to optimize direct regeneration method in Ziziphus jujuba Mill.
Materials and Methods: In this experimental study, the explants consisted of leaf cut into 3 parts, leaf cut from 4 sides, and full leaf of in vitro and compared in Murashige and Skoog and woody plant media with different concentrations of Thidiazuron (TDZ; 0, 5, 10, 15, and 20μM) and Naphthalene acetic acid (NAA, 0 and 1μM). The effect of 2 and 4 weeks of darkness on regeneration rate was investigated. The experiments were conducted in factorial based on a completely randomized design. The mean of statistical data was compared using Duncan’s multiple range test. SAS 9.3.1 software was used and the difference was considered significant at 1% probability level.
Findings: The 2 weeks of darkness treatment with the mean of 1.38 was better than the 4 weeks of darkness treatment. The Maximum number of shoots (2.27) was obtained in leaf cut into 3 parts. The maximum percent of regeneration (75.0%) and highest number of regenerated shoots (4.83) were obtained in the MS medium containing 10μΜ TDZ and 1μΜ NAA.
Conclusion: Regeneration rates in Ziziphus jujuba Mill is affected by the type of explant, culture media and plant growth regulators. Maximum rate of regeneration is observed in leaf cut into 3 parts and cultured on MS medium containing 1μM NAA and 10μΜ TDZ. Plantlets are rooted and successfully acclimatized at “in vivo” conditions.
Fereshteh Heidargholinezhad, Yousef Hamidoghli, Valiollah Ghasemiomran, Porya Biparva,
Volume 15, Issue 4 (10-2024)
Abstract
The production of secondary metabolites of medicinal plant
Volume 17, Issue 103 (September 2020)
Abstract
In order to investigate the effect of different levels of bentonite and pH of the extract on exposure to bentonite on the healing rate of stevia extract (Stevia rebaudiana Bertoni), a study was conducted on two factors in a completely randomized design with four replications. The first factor contains bentonite in four levels with concentrations of 0 (control), 10, 20 and 30 g / l and the second factor contains different pH of crude extract in five levels with 5.9 (control), they were 5.5, 5, 4.5 and 4, respectively. The results of analysis of variance showed that treatment with different amounts of bentonite had a significant effect on phenol, carotenoid, chlorophyll a, chlorophyll b and chlorophyll and flavonoid extracts. Also the best level of bentonite with the least amount and maximum clarification was 20 g / l with 0.07 mg / ml phenol, 0.36 mg / l carotenoid, chlorophyll a 1 mg / l, Chlorophyll b.03, Chlorophyll 3.03 mg / l and flavonoid content of 2.69 µg / ml were selected for subsequent experiment. The pH of the extract showed a significant effect on the phenol content of the extract and a significant effect on other traits. The best level of pH of the extract with the highest clarification and attention to human health, pH (5) with 0.15 mg/ml phenol, 1.42 mg / l carotenoid, 0.77 mg chlorophyll a Chlorophyll b 0.84 mg/l, chlorophyll b 1.61 mg / l and flavonoid with 2.17 µg / ml were selected. Therefore, the results of this study show that by considering appropriate amounts of dye compounds such as bentonite (20 g / l) at a suitable pH (5), the crude extract, color and transparency of stevia extract can be improved.
Volume 22, Issue 6 (11-2020)
Abstract
Froriepia subpinnata is an endemic endangered medicinal plant growing indigenous to Caspian coast forest in the north of Iran. This research was done to obtain primary information on morphological and phytochemical variation for the next step of domesticating and breeding of this plant. Twenty-three morphological traits measured indicated high variation among 52 accessions. There was a significant positive correlation between leaf dimension, plant height, and attributes related to biomass data. The range of phytochemical attributes analyzed was for total phenols (355.6-941.3 mg GAE 100 g-1 FW), total flavonoids (204.33-540.74 mg CAT 100 g-1 FW), total carotenoids (1.52 to 3.15 μg 100g-1 FW) and antioxidant capacity (31.36-81.82 DPPH%.). The HPLC analysis results showed that chlorogenic acid was the dominant phenolic compound generally found in this plant. Total carotenoids had no significant correlation with other biochemical traits, while the rest exhibited a linear relationship with each other. UPGMA cluster based on combined data of morphological and biochemical traits showed 3 distinct groups with high inter and intra-regional variations. Also, these information were combined with molecular data to separate environment and genetic effects. The high level of variation of phytochemical and morphological traits among and within populations could be a useful tool for future breeding and selection programs.
