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Showing 4 results for Hasanshahi
Volume 10, Issue 5 (Supplementary Issue - 2008)
Abstract
The present study was conducted to measure the level of job satisfaction among agricul-tural education teachers in Yazd Province of Iran. Agricultural education teachers from Yazd Province with a minimum of one year experience were included in the study. As a result so, the sample consisted of 60 agricultural education teachers. To study the job sat-isfaction level among respondents, the Brayfield and Rothe Job Satisfaction Index was used. A data form was used to collect information about demographic (independent) vari-ables and effective constraints reducing job satisfaction. The findings revealed that the majority of agricultural education teachers (56.7%) had a median level of job satisfaction, followed by 33.3 and 10 percent belonging to a low and high level of job satisfaction, re-spectively. According to regression analysis, "level of education", "number of students taught each year" and "class time (per day)" were found to have contributed to the in-crease in job satisfaction among agricultural education teachers. The effective constraints that had the most important impact on decreasing job satisfaction included an "absence of realistic expectations of teachers by society", "inadequate time for learning-by-doing programs" and an "inappropriate student-teacher ratio in class".
Zahra Hasanshahi, Behzad Dehghani, Tayebeh Hashempour,
Volume 13, Issue 3 (1-2023)
Abstract
Background:
HIV has at least six regulatory genes among which the Vif protein can control HIV replication. This study, as the first report, investigated the important mutations in VIF protein in sequences from Iranian patients and using immunoinformatics, conserved regions of this protein and B-Cell, T-Cell and CTL epitopes to stimulate the immune system, were determined.
Methods:
VIF sequences were obtained from NCBI GenBank, and tertiary structures, B-Cell, T-Cell and CTL epitopes were predicted by bioinformatics tools; besides, their antigenic and allergenic properties were studied.
Results:
The most prevalent mutations in Vif protein were related to S 49 P (90%), S 140 N and N 186 S (80%). Two substitutions at positions 41 and 42 were introduced which have effect on Vif binding to host factor. In addition, three regions were identified as the best epitope sequences with high potential to induce immune system and the lowest allergic properties, among which 5-32 region was suggested as the best vaccine candidate regions.
Conclusion:
This study as the first study from Iran using immunoinformatics tools to introduced a region with the high potential to induce humoral and cellular immune systems and lowest allergenic properties which can be used for further studies on HIV vaccines.
Volume 23, Issue 1 (Winter 2020)
Abstract
Aims: No definitive treatment or effective vaccine has been suggested against the East Respiratory Syndrome Coronavirus (MERS-CoV) virus which indicates the growing importance of the study of this virus. Amongst all MERS proteins, glycoprotein S has always been the main candidate for vaccine research against this virus, due to its function and structure. The aim of the present study was to investigate the structural, functional, and immunological properties of S protein using bioinformatics software that paves the way for designing an effective vaccine against this virus.
Materials & Methods: 35 glycoprotein S sequences of MERS were obtained from Genbank and amino acid changes were investigated. In addition, sequences were analyzed by various software for post-translational changes. Five types of software were used to evaluate the immunologic and allergenic properties. Finally, different structural aspects of this protein were predicted by SOPMA software.
Findings: The highest prevalence substitutions were found in amino acids of 95, 123, and 696 and the results indicate that there are four B-cell epitopes in glycoprotein S, and this protein has been affected by post-translational changes, including glycosylation and phosphorylation. This protein has no allergenic properties and the majority of its structure contains Alpha helix.
Conclusion: Glycoprotein S, especially in the RBD region of S1, has a high potential to induce the host immune system and the other features mentioned protein make it appropriate for the production of recombinant protein, including stability in host cells. Therefore, the use of glycoprotein S, especially S1, is recommended as a suitable candidate for vaccine design.
Volume 23, Issue 2 (Spring 2020)
Abstract
Aims: Many inhibitors have been introduced for the treatment of HIV-1 infections; however, most of these efforts have been failed due to the presence of resistant strains. The purpose of the current study was to investigate the treatment-resistance mutations in the HIV virus integrase gene and the effect of these mutations on the structure, function, and physical and chemical properties of this enzyme using bioinformatics software.
Materials & Methods: 36 HIV-1 integrase sequences form Iranian patients were obtained from the NCBI Genbank. After determining the mutations compared to the reference sequence, its post-modification and physical and chemical properties were described. Sequences subtypes, as well as the second and third structures, and possible interactions of this enzyme with the main inhibitors of the integrase were examined.
Findings: The analysis of selected sequences indicated a number of mutations in this protein. The subtype of most of the samples was A1 and the results of the analysis of the interaction showed that the mutations in the samples had no significant effect on the interaction of inhibitors with the integrase enzyme.
Conclusion: The binding site of these inhibitors is often found in the catalytic domain of integrase enzyme, and the results of this study depicted that most mutations were located outside this region, and this may be the main reason for the failure of these mutations to affect the interaction of inhibitors and integrase enzyme. Generally, the findings of this study suggest that anti-HIV inhibitors of HIV-1 can be used as an effective way to control this disease for Iranian patients.
