Showing 6 results for Hashemzadeh
R. Ghasemi , H. Hashemzadeh , H. Razavi , B. Yakhchali ,
Volume 9, Issue 1 (Winter 2018)
Abstract
Introduction: Growth hormone is a non-glycosylated polypeptide strand of the pituitary glands of all vertebrates that has a wide range of biological activities and considering the importance of this hormone and its importance and diverse therapeutic applications in medicine, its recombinant production can be of great importance. In recent decades, protein engineering and genetic engineering have resulted in a high level of expression and production of this protein in a variety of hosts, including Escherichia coli bacteria using new techniques and methodes, hormone purification and assay are carried out easily. Therefore, the aim of this review was to investigate the production of recombinant human growth hormone (rhGH) and future challenges.
Conclusion: One of the problems of the expression and purification of the human growth hormone may involve that maybe noted the production of inclusion bodies in the expression of recombinant proteins in the cell cytoplasm, the contamination caused by host proteins, low protein recovery from these inclusion bodies, low protein secretion into the Periplasmic space, high cost of production, especially in Purification stage and so on. Due to the lack of need for glycosylated hormone and high efficiency and simplicity of work, bacterial systems, especially Escherichia coli, are the most economical and effective systems for the expression of heterologous proteins. The hormone purification stage is usually the most costly process. Therefore, an optimal design for achieving the highest target protein recovery with the elimination of all contamination from the final product and reducing the purification step is required.
Z. Hashemzadeh , M. Jafarkhani Kermani , Y. Hamidoghli ,
Volume 9, Issue 4 (Fall 2018)
Abstract
Aims: The mass propagation and breeding new varieties of Ziziphus jujuba Mill as a valuable fruit tree and herb, which is well adapted to dry and semi-arid climate, is very important. The aim of the present research was to optimize direct regeneration method in Ziziphus jujuba Mill.
Materials and Methods: In this experimental study, the explants consisted of leaf cut into 3 parts, leaf cut from 4 sides, and full leaf of in vitro and compared in Murashige and Skoog and woody plant media with different concentrations of Thidiazuron (TDZ; 0, 5, 10, 15, and 20μM) and Naphthalene acetic acid (NAA, 0 and 1μM). The effect of 2 and 4 weeks of darkness on regeneration rate was investigated. The experiments were conducted in factorial based on a completely randomized design. The mean of statistical data was compared using Duncan’s multiple range test. SAS 9.3.1 software was used and the difference was considered significant at 1% probability level.
Findings: The 2 weeks of darkness treatment with the mean of 1.38 was better than the 4 weeks of darkness treatment. The Maximum number of shoots (2.27) was obtained in leaf cut into 3 parts. The maximum percent of regeneration (75.0%) and highest number of regenerated shoots (4.83) were obtained in the MS medium containing 10μΜ TDZ and 1μΜ NAA.
Conclusion: Regeneration rates in Ziziphus jujuba Mill is affected by the type of explant, culture media and plant growth regulators. Maximum rate of regeneration is observed in leaf cut into 3 parts and cultured on MS medium containing 1μM NAA and 10μΜ TDZ. Plantlets are rooted and successfully acclimatized at “in vivo” conditions.
H. Hashemzadeh, A. Allahverdi , P. Ertl, H. Naderi-Manesh,
Volume 10, Issue 4 (Fall 2019)
Abstract
In view of the constant increase of nanotechnology and nanomaterials applications in our daily life, to determine whether they are safe, “in vitro” and “in vivo” screening methods are needed. Obviously, application of models that are similar to the physiological tissues process of the human body could be a better candidate. The three-dimensional spheroid method, spheroid were generated using commercial microplates, has many benefits (in comparison with traditional methods or monolayer cell culture) such as the growth of the cells in 3D, similar to the body's physiological tissue, an alternative for animal models, cell-to-cell interactions, and better cell signaling. In this study, the toxicity of silver nanoparticles by using three factors such as metabolic activity, live/dead assay, and spheroid surface area was evaluated using two different methods (2D vs 3D) under treatment with various concentrations of silver nanoparticles at different times. The results showed that different cells types, cancer and/or normal lung cells, have significant differences. In addition, it was observed that distinct differences in terms of cytotoxicity of silver nanoparticles between 2D and 3D culture systems and also the rate of growth/non-growth of spheroids are highly depended on cell type and various concentrations have fundamental importance in such studies. The present study provides evidence that cellular dimensions (3D vs 2D) play a pivotal role in the results and outcomes of inflammation and cytotoxicity with nanoparticles due to the spatial-temporal structure.
J. Esfandyari, B. Shojaedin-Givi, M. Mozafari-Nia, H. Hashemzadeh, H. Naderi-Manesh,
Volume 10, Issue 4 (Fall 2019)
Abstract
Diatoms biosilica shell, frustule, is substitute biostructures to mesoporous silica particles, which possesses their wide surfaces, nano-diameter porosity, mechanical strength, and thermal stability, optical capabilities, and the ability to bind to biomolecules can be used in biosensing applications. In this study, diatom species called Chaetoceros muelleri, was used for the fabrication of the Fe2O3-Au-Biosilica magnetic package. After micro-algae cultivation, the synthesis of gold nanoparticles (AuNPs) on silica walls was carried out using the bio-synthesis method, which evaluations have demonstrated the continuous formation of spherical AuNPs on the walls and its surfaces. After this step, the magnetic iron oxide nanoparticles were attached to the silica surface of the diatom, this, in turn, leads to system guiding using a magnetic field. Surface modification of diatoms magnetic complex, by using the APTES, allowed the attachment of fluorescence Rhodamine and the Herceptin antibody (Trastuzumab) to the structure. As well as the attachment of the fabricated system to target cells (SKBR3) was confirmed by fluorescence microscopic analysis. The results of this study indicate the ability and specificity of the diatom silicone shell as a "multipurpose" package for diagnostic and therapeutic activities.
Volume 12, Issue 4 (Number 4 - 2010)
Abstract
The effects of various methods of probiotic administration in hatchery and on prevention of Salmonella enteritidis (Se) in broiler chicks was investigated. A total of 150 Salmonella free day old chicks (Ross 308) were assigned to five experimental groups including control and four in-hatchery probiotic administration method groups comprised of: in ovo injection, oral gavage, spray and vent lip application. Each group was comprized of 30 chicks. The chicks were challenged by 8 Log CFU Se using oral gavage on 2 days of age. At 1 and 7 days of post-challenge (PC) 15 birds per experimental group were sampled for Se recovery through either one of culture or culture based PCR techniques. Administration of probiotics reduced the number of Se colonized chicks, compared with control as evaluated through either culture or PCR method. These reductions were significant for all the administration routes (P< 0.05), except for the 1 day PC, evaluated by culture method (P> 0.05). Furthermore probiotics were capable of reducing the number of colonized chicks from day 1 to day 7 PC. Vent lip method was evaluated as the most effective route of probiotic administration in prevention of Se colonization, not significantly different from either spray application in day 1 of PC group or from other administration methods in the day of 7 PC (P> 0.05). PCR method was more reponsive in detection of Se as compared to traditional culture method. Administration of probiotics in hatchery finally resulted in reducing the colonization of Salmonella in the alimentary tract of chicks.
Volume 17, Issue 2 (3-2015)
Abstract
Lactobacilli, like the other gut commensal bacteria, are well known for their use in poultry nutrition and for their probiotic properties. However, little is known about their interaction with the gastro-intestinal tract when administered in vivo. To specifically monitor the passage of lactobacilli after administered in hatchery, Lactobacillus plantarum was transformed with the recombinant vector pLEM415::gfp. A total of 200 one-day old chicks (ROSS 308) were assigned to five experimental groups including the control and four in-hatchery probiotic administration method groups comprised of in ovo injection, oral gavage, spray, and vent lip application. At 0, 4, 12, 24, 48, and 72 hours post-probiotic administration, 6 chicks were sacrificed from each group. Adhered bacteria were sampled from intestinal sections. Polymerize chain reaction (PCR) was used to trace the transformed L. plantarum in the alimentary tract of the birds. The GFP transformed bacteria were detected in intestinal samples of oral gavage, spray, and in ovo injection groups; while in vent lip method no GFP transformed bacteria were detectable. Oral gavage method of probiotic administration was the most effective route, which seemed to be the result of direct delivery of the full dose of probiotic microorganisms into the target sites. Based on the results of this trial, administration of probiotic in hatchery had a positive effect on the morphology of the intestine and in ovo injection route, and oral gavage method seemed to be more effective. In this experiment, the utility of transformed probiotic bacteria with GFP was shown to monitor the fate of the probiotics when administered via various routes to poultry.
