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Pseudomonas aeruginosa is one of the most important causes of infection in medicine, which in recent years is known as an antibiotic resistant bacterium. One of the antibiotic resistance strategies of this bacterium is algD and PpyR genes expression for biofilm formation.

 In recent years, it has been shown that using microorganisms, such as probiotics, is a method of pathogen bacterium harnessing, hence, in this study, for preventing biofilm formation of P. aeruginosa, E.coli Nissle 1917(EcN) probiotic bacterium is used, as a new treatment choice.

Due to direct relationship between antibiotic resistance and biofilm formation, strains with the    

highest antibiotic resistance was chosen by antibiogram test. Then, in order to determine the inhibition rate of EcN bacterium in the formation of biofilm caused by P. aeruginosa bacterium, a biofilm formation test was performed.

At the end, to evaluate algD and PpyR genes expression, which were key parts of biofilm formation, in the presence of probiotic EcN bacterium, Real- time PCR method was used.

Based on the results of the biofilm formation test, EcN bacterium showed a high inhibitory effect on the formation of biofilm caused by P. aeruginosa bacterium.

.Also, in assessment of algD and PpyR genes expression in presence of EcN probiotic, a significant reduction in PpyR gene expression has been seen, in comparison with control group. The results of this study showed that EcN probiotic can act as a suitable new treatment option, to reduce P. aeruginosa biofilm formation.

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In this paper, the behavior of curved sandwich beam with a soft flexible core, under low-velocity impact, loaded with environmental thermal effects by pursuing the use of the high order shear deformation theory of sandwich structures is investigated. The Sandwich beam is comprised of composite sheets and foam core. The boundary condition is simply supported by probability of circumferential deflection. Two degrees of freedom for mass- spring model was used for modeling the impact phenomena. In the presented formulation, the first order of shearing deformation theory is used for sheets,the core Displacement field is considered unknown and then by using elasticity theory and compatible condition in the core, sheets common face and the relation of stress-strain core deflection are determined. In order to derive the governing equations of beam structure, the Hamilton principle was used. For validation, the results obtained from this research are compared with the results of other researchers and also the numerical result of ABAQUS software. The comparison of results shows good agreement. The effects of various parameters like impact velocity and mass, environmental temperature, core and sheets thickness and materials on core and sheets deflection and core stress and impact force were studied. The obtained results showed that increasing environmental temperature has a slight effect on impact force, but more effect on beam dynamic response. It is also shown that with increasing the hardness of beam, the energy absorption is reduced.