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Showing 2 results for Jaberi Ansari
Farshid Jaberi Ansari, Zahra Hajihassan, Hasan Jalili,
Volume 6, Issue 2 (11-2015)
Abstract
Production of recombinant proteins such as β-NGF using prokaryotic hosts is the topic of many recent researches. However, bacterial cell culture media are cheaper than eukaryotic cell culture media, but in industrial production scale they are not cost effective at all for biotech companies. Therefore, survey to find inexpensive cell culture medium that bacterial cells not only can grow in it but also produce recombinant proteins is very important. In this study, for the first time date syrup and yeast extract mixture was used as an inexpensive medium. In RSM (response surface methodology) studies different concentrations of date syrup and yeast extract were used as carbon and nitrogen sources respectively. The results indicate that the 20 g/lit of carbon and 5 g/lit of nitrogen are optimum for bacterial growth. Also the data show that recombinant bacteria not only can grow but also can produce recombinant proteins such as β-NGF using this synthetic medium.
F. Jaberi Ansari, H. Jalili ,
Volume 9, Issue 4 (Fall 2018)
Abstract
Aims: One of the ways to reduce cholesterol is to use statins that prevent cholesterol synthesis. The statins are similar to mevalonate and act as a competitive inhibitor of HMG-CoA reductase enzyme. Lovastatin is the eminent derivate of the statins group, which is produced by many microorganisms. At commercial scale lovastatin is produced in submerged culture by Aspergillus terreus. The industrial production of this metabolite is carried out by Aspergillus turosus in liquid culture. The main aim of this research was to investigate the effect of spore age on lovastatin production at the inoculation stage; also, the impact of adding olive oil and tetracycline as inducers for lovastatin production were examined.
Materials and Methods: In the present experimental research, different suspensions from varying ages of spore were prepared and added to the medium of Aspergillus terreus ATCC 20542; lovastatin concentration also was measured by High Performance Liquid Chromatography (HPLC).
Findings: The utmost lovastatin was observed in inoculum with 85 days spore age and equal to 60 mg/l, which was approximately twice higher compared to when inoculated with 10 days spore age. The best concentration of spore inoculation was 0.5×107 spores/ml. Lovastatin production significantly increased when tetracycline and olive oil were used as inducers.
Conclusion: As the inoculated spore age increases, lovastatin and biomass production is increased. The lovastatin production is increases by more than 1.5 times while adding tetracycline and olive oil compared to date syrup alone.
