Search published articles
Showing 2 results for Jahazi
Sheyda Jahazi, Hashem Yaghoubi, Hossein Akbari,
Volume 11, Issue 2 (Spring 2020)
Abstract
Iron oxide nanoparticles are one of the nano carriers that are suitable for novel drug delivery systems due to low toxicity, biocompatibility, loading capacity and controlled drug delivery to cancer cells. The purpose of this study is the synthesis of coated iron oxide nanoparticles for delivery of Doxorubicin (DOX) and its effects on cancer cells.
In this study, Fe3O4 magnetic nanoparticles were synthesized by Polyol method, and then doxorubicin was loaded onto PEGylated iron oxide nanoparticles. FT-IR was used to ensure PEG binding to nanoparticles and loading the drug onto nanoshell. Comparison of the mean size and the crystalline structure of nanoparticles were performed by TEM and X-ray diffraction pattern. Then, the effect of cytotoxicity was evaluated on AGS and MCF-7 cancer cells by MTT assay.
According to FT-IR results, the presence of O-H and C-H bands at 2927 cm-1 and 3392 cm-1 peaks correlated with PEG binding to nanoparticles. XRD pattern showed the cubic spinel structure of trapped magnetite nanoparticles carrying medium with a mean size of 14 nm. 21.67% of Doxorubicin was loaded into Fe3O4-PEG nanoparticles, which the highest drug release recorded during the first 24 hours. MTT assay at 24, 48 and 72 h treatments showed that with increasing concentrations of doxorubicin loaded Fe3O4-PEG nanoparticles from 0 to 50 μm, the cytotoxic effects of the drug gradually increase.
This study showed that PEGylation of iron oxide nanoparticles and using them in drug delivery process to increase the effect of Doxorubicin on AGS and MCF-7 cancer cells
Volume 23, Issue 1 (Winter 2020)
Abstract
Amis: In recent years, carbon nanotubes have attracted the attention of many researchers because of their unique properties. In the present study, carbon nanotubes were coated using PEI. Then, their ability to gene delivery to E. coli cells was examined.
Materials & Methods: Nanotube- PEI nanoparticles were synthesized by the reaction between amine groups of PEI and carboxyl groups of nanotubes. In order to prepare the appropriate DNA vector for delivering to E. coli cells, the Gus A gene was transferred from pBI121 to PUC18 vector (pUC-Gus). Nanotube-PEI/DNA complexes were prepared by combining different mass ratios of nanotube-PEI (0.5, 1, and 2 w/w%) with the fixed amount of DNA. To the transformation of E. coli, the appropriate amount of nanotube-PEI/DNA complexes was added to E. coli cells under stirring at 37°C for 7h. The transformation efficiency of E. coli was determined by colony counting on LB agar supplemented with Ampicillin. Moreover, Gus staining assay was used to confirm the function of the plasmid. Determination of cytotoxicity of nanotube-PEI was performed using MTT assay at 6, 24, and 72 hours intervals at different concentrations of nanotube-PEI (10, 100, and 500μg/ml).
Findings: The nanotube-PEI was synthesized successfully. Nanotube- PEI nanoparticles have a great ability to protect DNA from enzymatic digestion. The percentage of E. coli cells viability was decreased by increasing both the concentration of nanotube-PEI nanoparticles and also the duration of incubation. The results of the agarose gel electrophoresis of plasmid extracted from E. coli and digested using EcoRI enzyme showed that the pUC-Gus plasmid has been successfully transfected by nanotube-PEI nanoparticles to E. coli bacterial cells.
Conclusion: Cationic carbon nanotubes have a high ability to gene transfer to E. coli.
