Showing 3 results for Javani Jouni
J. Zafari , F. Javani Jouni , M. Satari Keykaleh , P. Abdolmaleki , M.j. Khodayar , A. Jalali ,
Volume 9, Issue 4 (Fall 2018)
Abstract
Aims: Regarding the treatment of cancer, due to the limitation in the use of high dose and resistance of cancer cells, it is necessary to use optimal methods that have high therapeutic efficacy and reduce the dose of radiation and medicine. The aim of the present research was to investigate toxicity of cisplatin under the influence of static magnetic field in susceptible and drug-resistant cell.
Materials and Methods: In the present experimental study, A2780-CP resistant cell classes and susceptible to A2780 cisplatin were investigated in the field and drug-treated cell groups compared to the drug-receiving group alone, and to determine the effect of static magnetic field and concentration of drug, 10mT for 24 hours and logarithmic drug concentration (1, 10, 50, 100, and 500mcg/ml) were used. Inhibitory concentration of 50% cell growth (IC50) was obtained for the cells in the absence and presence of the magnetic field after conversion of the absorption obtained in the ELISA from the MTT test to cytotoxicity percentage. Data were analyzed with Prism software using two-way ANOVA and T-test.
Findings: In the presence of a static magnetic field and different drug concentrations, a greater reduction in the percentage of In vivo cells was observed. IC50 values for A2780 cells in the absence and presence of magnetic fields were 27.69±9.58 and 8.96±1.48μg/ml for A2780-CP, and 61.61±8.03 and 9.58±3.13μg/ml, respectively.
Conclusion: The mortality rate of the cells treated with cisplatin under the influence of the magnetic field is more in susceptible and drug-resistant cells than that of only drug use. Drug-resistance decreases in the drug-resistant cell class in the presence of a magnetic field.
Volume 16, Issue 3 (12-2013)
Abstract
Objectives: This study investigated the possible synergistic effect of simultaneous treatment of bone morphogenic protein (BMP)-4 as a chemical stimulator and static magnetic field (SMF) as a physical stimulator on viability percent and proliferation rate in rat bone marrow stem cells.
Methods: Passage 5 cells were trypsinized, and a cell suspension prepared after which the cells were counted and cultured in 25 cm2 flasks. Cells were incubated for one day and washed with phosphate-buffered saline. We added BMP-4 at the optimum concentration of 25 ng/ml at different times (24, 48 and 96 h) into the medium. The cells were exposed at an optimum intensity of 4 mT of the SMF at different exposure times (24, 48, and 96 h). Subsequently cells were washed with phosphate-buffered saline, trypsinized, and separate cell suspensions were prepared from each flask. We investigated the viability and proliferation rates of treated cells by staining them with Trypan blue and performed cell counts with an optical microscope. The mean numbers of whole cells and living cells were considered to be the proliferation and survival rates, respectively.
Results: Increased SMF exposure and BMP-4 increased the viability percent and change in proliferation rate in the treated groups compared with their corresponding controls. The maximum increased viability was observed in the group that was treated with BMP-4 for 96 h.
Conclusion: Our results have supported the hypothesis that SMF alters the viability and proliferation rate of treated BMSCs, which was enhanced when the cells were treated simultaneously with SMF and BMP-4.
Volume 20, Issue 2 (8-2017)
Abstract
Objective: Despite significant advances in radiation therapy and cancer treatments in the past 30 years, resistance to chemotherapy is a major obstacle in the recovery of patients with cancer. Resistance to chemotherapy drugs inhibits the recovery process. This study aims to evaluate the anticancer activity of the methanolic extract of rosemary alone and in conjunction with gamma rays on growth inhibition of MCF-7,SKBR3 and Huo2 cell lines.
Methods: We examined cytotoxicity of different concentrations (1, 5, 10, 50, 100, 500 μg/ml) of rosemary extract and various doses of gamma rays (1.5, 3, and 7.5 Gy) on MCF-7, SKBR3, and fibroblast (HU02) cell lines. All three cell lines were obtained from the Iranian Biological and Genetics Reserve Center. The cell lines were grown in DMEM supplemented with 10% FBS, 1% penicillin and streptomycin. The cells were allowed to incubate at 37ºC in an atmosphere that contained 5% CO2 and 100% humidity. The standard MTT assay was performed to estimate cell viability after treatment with gamma rays and rosemary extract.
Results: The results of the MTT assay showed that rosemary extract had time- and concentration-dependent anticancer activities on the MCF-7 and SKBR3 cell lines (p<0.01). Rosemary extract had no remarkable cytotoxicity on the normal HU02 cell line. Gamma rays along with rosemary extract had more cytotoxic activity in a time-dependent manner on viability in the cancer call lines (p<0.03).
Conclusion: Our results, along with results from other studies, have suggested that rosemary extract is a potential candidate, either alone at pre-determined doses or in combination with gamma rays, for the treatment of chemotherapy resistant breast cancer.
