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A great part of sciences and fundamentals of formation of our cities and past architecture has been destroyed which rooted in regulation of human relationship with the environment. Architects and urban planners deal with their processes only based on modern conditions and fundamentals that are general and international. Lack of these sciences in architecture as well as urban planning has separated it seriously from tradition and finding these fundamentals may, despite of benefitting from modern civilization values, cause biological and health values to be continued based on traditional system in modern architecture and urban planning. Plenty of contemporary cities that recently have been located and constructed are facing with problems in terms of benefitting from natural elements and based on health indicators in the tradition. New academic architecture and urban planning system has trusted in new rules of adjustment of environmental conditions to the extent that even is not seeking for traditional climatic criteria and indicators and is not supposing that many of these problems are arising out of inattention to this traditional science. In the meantime, wind as a climatic-traditional element has direct impact on health of residents of a city. The winds are crucially effective on filtration or creation of pollution in a city. Studies indicated that the wind due to direct impact on human health and is a superior element in prosperity and structure of a city and vernacular architecture of each region, and has a crucial role in filtration or creation of pollution in a city. In this paper, the impact of wind as a natural element on human’s health has been analyzed by considering traditional medical texts and its impact on selection of place and structure of cities has been studied. For this purpose, after analysis of religious, philosophical and medical texts, and description of priority of winds in relation to two factors of wind direction and wind origin, six climatic zones of some of old, important and populous cities were ranked in the light of existence or absence of favorable and unfavorable winds, respectively. Then, by studying the structure of a sample of cities with favorable wind and a sample with unfavorable wind, solution for traditional cities in terms of benefitting from/coping with the wind has been studied. Analysis method used for Islamic and medical fundamentals of wind is interpretive and argumentative. The criterion for selecting cities is being old and populous and located in six climates of Iran. Thereafter, selected cities were classified and graded based on wind quality and considering theoretical fundamentals. The basis of identification of prevailing wind direction in cities includes new climatic books, statistics of meteorological stations as well as internet. Based on fundamentals of traditional and Islamic medicine that is a little different from Greek medicine, human skin breaths in good weather and becomes fresh and in case of lack of wind passage, still air make it stink and putrid. Still air is putrefied and putrefied air is infected. Yet, unsuitable winds may destroy the body’s vitality and be detrimental to health as well as impose bad impacts on human psyche. In nature, life of plants and animals is regulated by wind so that in Quran, it has been mentioned repeatedly and swore spiritually. In the medical fundamentals, wind types and its impact on human health and climate has been studied. According to analysis of Islamic and traditional medical fundamentals, winds quality, from the most favorable to the unfavorable ones respectively, includes east wind, north wind, west wind and finally south wind; meanwhile, in addition to the importance of wind direction, wind origin is also important for its impact. Wind origin includes environments such as sea, desert, mountain, plain, lagoon and forest. In Iran, due to diversity of its climate, instances of each one (usually the first four cases are more evident) can be taken into consideration. Removal of unfavorable wind and attraction of favorable wind in the city creates strategies for city structure. Environmental factors such as wind are significantly important for locating cities. In different regions of Iran, the wind is blown in different directions and from various origins. Each city tries to receive and train any favorable wind. In this study, six climatic zones were selected and in each climate two cities, one with favorable wind status and another one with unfavorable wind blowing were studied to compare the appropriateness of model and experience of ancients for benefitting from or coping with the wind, regarding the health criteria from Islamic viewpoint. In Iranian urban structure model, spatial orientation methods of Iranian housesthat is called Ron (direction) in traditional architecture, had been converted into a model for design of that time. Three fundamental Rons in this regard include Rasteh Ron, Esfahani Ron, and Kermani Ron. Rasteh Ron is referred to the northeast-southwest direction. In this direction, the inside of house is cold in winter and hot in summer. Esfahani Ron is referred to the northwest-southeast and Kermani Ron is referred to east-west direction. In Vitruvius book, an eight-direction model has been introduced for main directions of winds and their specifications that are exposed to main and secondary directions (90 and 45 degrees). Whereas general principle in his proposal is avoiding the same direction with winds in the city passages, so axes shall not be located in main and secondary axes. Iranian Rons, except Kermani Ron, are not located in main and secondary directions and Kermani Ron has been created with a little deviation of the skyline (almost 20 degree); therefore, main lines (including passages and orientation of buildings) of no city are located in main and secondary directions of wind flow. Considering the medical fundamentals of wind direction and superiority of north and east winds to west and south winds in all three Iranian Rons, tendency toward receiving north wind and rejection of south wind such as building backward to the south front can be seen, but in Rasteh and Kermani Ron, tendency for acceptance of east wind and in Esfahani Ron, acceptance of west wind is seen as well. In fact, if a wind of west direction is a favorable wind considering wind origin, the city’s orientation structure is set according to Esfahani Ron to benefit from favorable west wind, while this Ron has less frequency than Rasteh Ron. For instance, in cold climate, mountains of Shahrekord are located in the south that is origin of prevailing wind, so the city has the most unfavorable wind and mountains in the south prevent its suitable sunbathing; thus, its direction tends to the west (Esfahani) so that relative heat of west wind may adjust the coldness of city for the residents. Analysis of instances shows that urban structure of ancient cities has been completely formed based on medical fundamentals and is appropriately conformed to the health factors from Islamic viewpoint. Comparison between quaintly and priority of traditional urban Rons and observance of priority of benefiting from east and north winds and appropriate angle in most of them is the best evidence for paying attention to health in old cities of Iran. Considering the adaption of the past urban structure and health factors from Islamic perspective may be applied on contemporary urban planning and underlie for improvement of environmental pollutions.

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The present study was accomplished to purify and biochemically characterize the phenol-degrading enzyme from the bacteria existed in petroleum-contaminated soils. The catechol 1, 2 dioxygenase was extracted from Aneurinibacillus migulanus Isolate ZNU05 and purified using Q-Sepharose ion exchange chromatography column. The enzyme activity was examined under different pHs (ranged from 4 to 9), at different temperatures (ranged from 20 to 70˚C), in the presence of various metal ions chloride salts (Ca²⁺, K⁺, Mn²⁺, Co²⁺, Zn²⁺, Mg²⁺, Cu²⁺ and Na⁺), and with various solvents (ethanol, ethyl acetate, petroleum ether, acetonitrile, N-amyl alcohol, N-hexane, and toluene). In addition, the enzyme activity was investigated using different substrates such as phenol, catechol, benzoic acid, pyrogallol and α-naphtol. SDS-PAGE analysis indicated that there was a single-band protein with a molecular weight of approximately 40 kDa. The catechol 1, 2 dioxygenase had a maximum activity at temperature 30˚C at pH 8.5. Moreover, the catalytic activity of the enzyme was increased in the presence of cobalt and zinc ions as well as organic solvent of amyl alcohol, while it was decreased or inhibited in the presence of the other metal ions and organic solvents used. Among different substrates on enzyme activity, catechol was the most favorable for the enzyme, so that, the V_max and K_m were 8.959 U/mg and 4.992 µg/mL for the substrate, respectively.

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Nowaday, product quality of industrial factories ,has become the challenge for their market share in competitive world .Design of experiment (DOE), as one of the newest methods of improving quality, defines the significantly effective factors and sets their optimum levels by running the experimental design. Among all, the fractional factorial designs runs, have priority to others because of the decrease in the number of In Iran, Despite its disability in resolving interaction effects,Taguchi design as the fractional design subgroup, has been used sofar for its easy analysis. In this article, a fractional design with high- resolution degree, was defined as a case study on the refractory brick quality in Pars Refractory factory. To reach this target, the fractional design 25-1 with the high resolution degree of V,was designed and run. The results showed that controlling and setting the main interaction effects in the optimum level, lead to the minimum operation time and 41% increase in product quality. Research and development units can cause promotion in the quality of products by implementing fractional designs with suitable resolution degree and analyzing the main and especially interaction effects.

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Objective: The LRR (leucine rich proteoglycans) is a molecular recognition motif found in proteins with some roles in cell adhesion, signal transduction, DNA repair and RNA processing. Opticin is a member of this family. Takanosu et al (2001) detected messenger RNA expression of mouse opticin in the eye, heart, brain, testis, thyroid and epididymis by dot blot hybridization. In this study, expression levels of mRNA and protein of opticin was investigated by two monoclonal antibodies which were raised against opticin peptides. By this method structure of opticin in human and mouse has been studied. Materials and Methods: Mouse tissues including, kidney, testis, liver, lung, heart, brain, muscle, spleen and eye were isolated. Opticin expression was identified at mRNA and protein levels by RT-PCR and Western blot. Results: PCR analysis revealed that opticin mRNA is expressed in all the tissues studied except for the lung. However, opticin protein was detected in all tissues analyzed. Conclusion: Expression of opticin in the adult murine tissues may suggest functions other than that of putative regulation of vitreous collagen fibrillogenesis for this molecule.

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Objective: Cell vital function has correlation with mechanical loadings that cell experiences. Here, effects of in-vitro combined cyclic-static stretch on proliferation of human mesenchymal stem cell (HMSC) were evaluated. Materials and Methods: HMSCs were cultured on gelatin coated elastic membranes, and exposed to stretch loading. Four different regimes of cyclic, static, combined cyclic-static, and cyclic with a period of unloading were exerted on the elastic membrane. Duration of cyclic loading and static loading was 5 and 12 hours respectively. Results: The results illustrate that 10% cyclic stretch causes cell alignment but there were no significant proliferation differences between control and test group. Combined cyclic-static stretch reduced proliferation significantly while cyclic stretch with an unloading period increased cell proliferation significantly. At last, static stretch did not affect cell proliferation significantly. Conclusion: Cell stretching regimes and post-loading duration are effective factors on cell proliferation.

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A hexapod machine tool with a parallel structure has six degrees of freedom. This machine has a high dexterity unlike traditional machine tools. The hexapod can be used in machining free form surfaces. Free form surfaces are widely used in today industries. These surfaces are much encountered in auto, aerospace and mold design industries. Therefore machining of these surfaces has attracted the attention of researchers. In this field much research has been done in five axis machine tools. In this paper machining free form surfaces with hexapod machine tool has been investigated. The main topic of this paper is the feasibility of using hexapod as a machine tool table and machining with it. First, the interpolation of free form surfaces for parallel structure machines is explained. Then NURBS curves and surfaces are described and its formulation in matrix form is explained. Then extracting information of free form surfaces with NURBS formulation is explained. Subsequently, some explanation about preparation of machining is given. Finally two free form surfaces designed in Catia and have been machined with the developed hexapod machine tool.

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Jam is a product that uses a lot of sugar in its preparation, and this compound plays an important role in increasing the level of sugar. Since the increase in blood sugar is associated with diabetes and obesity, so reducing sugar consumption in this product can reduce the risk of these diseases which are caused by long-term use of this product. In this study, enzymatic invert sugar was used as a substitute for sugar in different ratios (0, 25, 50, 75 and 100%). Quince seed gum and pectin were also used as thickeners to improve the texture properties of jam. Measurement of acidity and pH, viscosity, brix, total sugar, reducing sugars, energy intake and sensory properties were measured. According to the results, with adding the gum and pectin, pH decreased, followed by acidity, brix, viscosity, reducing sugars, tissue hardness, yellowness and lightness index as well as sensory properties scores. Also, with increasing invert sugar, the acidity of samples increased, while brix and viscosity of samples decreased significantly (P˂0.05). But as the invert sugar concentration increased, the taste and color scores of the samples increased. The color score of the samples was significantly increased (P˂0.05) by adding quince seed gum. There was no statistically significant difference in odor of samples with increasing sugar replacement percentage (P≥0.05). In general, based on the results of physico-chemical and sensory tests, it can be said that jam samples containing 75% invert and 0.25% of gum to (treatment number 15) were the best samples with 15% fewer calories than control samples.

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The purpose of this study is to investigate the ability of smart scaffolds of Kappa-carrageenan (Carr) and the combination of Kappa-carrageenan and quince seed mucilage (Carr:Quc) to support C2C12 viability and growth for cultured meat production. Carr and Carr:Quc with a final concentration of 1.5% (v/w) were developed using a 5% potassium chloride solution. The capability of the scaffolds to respond to the pH change of the environment was evaluated, and the viability of C2C12 at normal pH (7.4) and varying pH levels (7.4-5.5) was assessed. The evaluation of swelling changes with varying pH (pH 1-7) showed that for the Carr scaffold, the highest swelling was observed at pH 5, reaching 145%, which showed a significant difference compared to swelling at other pH levels (p < 0.05). The highest swelling for the Carr:Quc scaffold was also observed at pH 5, reaching 428%, with a significant difference compared to swelling at other pH levels (p < 0.05). Moreover, the change in the swelling behavior of the scaffolds was evaluated by changing the pH from 7.4 to 5.5. Carr did not show any swelling change, while Carr:Quc demonstrated a significant change in swelling after exposure to pH 5.5 for 30, 45, 60, 180, and 360 min. On Carr:Quc, C2C12 showed higher viability in normal conditions compared to varying pH levels from 7.4 to 5.5. Furthermore, after culturing on Carr:Quc, C2C12 maintained their viability throughout the culture period for 15 days at pH 7.4 and showed the potential for spheroid formation. The findings of this study could pave the way for the design of scaffolds made of edible biopolymers to facilitate tissue engineering of cultured meat