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Aims: The induction of artificial over-expression of miRNAs is an appropriate approach to more effective cell differentiation. The significant role of microRNA-1(miR-1) has been reported in the development and differentiation of cardiac cells. Lentivirus is an effective vector for stable cell line production. The aim of this study was the production of recombinant HEK293T with miR-1 overexpression as a biological model for cardiac studies.
Materials & Methods: In this experimental study, HEK 293T cells were cultured in DMEM medium with 10% Fetal Bovine Serum (FBS) and L-glutamine 2mM and Penicillin-Streptomycin 1X in incubator medium. After cloning of miR-1 gene, recombinant clones were selected and the recombination was confirmed by sequencing. The miR-1 carrying vector and auxiliary vectors were packaged in the HEK293T to produce the recombinant virus. The infection of HEK293T by recombinant virus was performed in order to achieve stable cell line. Then, GFP fluorescent marker evaluated the efficiency of transfection and effective virus dilution. Finally, the alteration in expression level of miR-1 was assessed by qPCR. Data analysis was performed by comparing the threshold cycle and Pfaffl method.
Findings: The most GFP expression was detected in transfected cells by 150 micromole dilution. GFP fluorescent marker facilitated optimization and purification of recombinant cells. qPCR investigation demonstrated the significant increase in expression of miR-1 in transfected cells in comparison to controls.
Conclusion: The stable recombinant HEK293T miR-1 over-expressing cell line in lentivirus can be utilized as a suitable biological model for investigation of cardiac evolution and development processes.

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Aims: The moral sensitivity of nurses is related to patient care. The more sensitive nurse in facing ethical challenges in care causes the better patient care he/she provides. Providing better ethical care leads to increased patient satisfaction and reduced societal burden. We investigated the relationship between nurses’ moral sensitivity and patient satisfaction in the Iraqi emergency wards.
Instrument & Methods: This cross-sectional study was done on nurses (200) and patients (400) in Iraqi emergency wards. Three questionnaires were used, including demographic, Newcastle Satisfaction, and Moral Sensitivity. Data were analyzed by SPSS version 20.
Findings: There was a positive relationship between patient satisfaction and nurses’ moral sensitivity (r=0.662; p=0.001).
Conclusion: Nurses often face difficult situations when caring for patients in the emergency department. To deal with these situations and make ethical decisions, nurses must have an acceptable level of ethical sensitivity. When nurses are ethically sensitive to patient care, patients realize this, and satisfaction with receiving nursing care increases.
 

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Aims: This study compared two groups of twin pregnancies complicated by twin-to-twin transfusion syndrome (TTTS), with and without selective fetal growth restriction (sFGR), and evaluated the survival rate at 30 days after birth treated with fetoscopic laser photocoagulation (FLP).
Materials & Methods: The present study was a retrospective study of 164 diamniotic monochorionic twin pregnancies complicated with TTTS and treated with FLP. The sFGR was defined as an estimated fetal weight below the 10th percentile. The independent t-test, Chi-square test, or Fisher's exact test were used for statistical analysis. Multiple logistic regression analysis was performed to identify 30-day donor twin survival risk factors for the entire study population. The significance level was determined at p<0.05.
Findings: Of the studied cases, 45.1% had only TTTS, while 54.9% had both TTTS and sFGR. .There was a significant difference (p<0.001) in the distribution of Quintero stages and maternal age during the intervention (p=0.01) between the two groups. The sFGR before laser surgery in TTTS patients was associated with reduced donor survival. The multivariate analysis revealed that gestational age at delivery (OR=0.81, 95%CI:0.7-0.8) and sFGR (OR=0.43, 95%CI:0.2-0.8) were significantly associated with donor survival.
Conclusion: The sFGR before FLP is present in approximately 55% of TTTS cases, which is caused by normal placental abnormalities. TTTS with sFGR is associated with reduced donor embryo survival. Gestational age at delivery and sFGR are important factors affecting donor survival 30 days after birth. Performing successive ultrasounds after diagnosing monochorionic twin pregnancies is essential for timely identification, correct management, and treatment.