Showing 8 results for Satari
L. Satari Faghihi, S. Ahmady-Asbchin, B. Seyedalipour , Gh.r. Riazi ,
Volume 9, Issue 3 (Summer 2018)
Abstract
Aims: Today, the ability to produce hydrolases enzyme that are active in high salt concentrations is considered a new approach to the use of halophilic bacteria in biotechnology. The aim of this study was the screening and isolation of extracellular lipase producing halophilic bacteria Marinobacter sp. S-14 isolated from Badab-e Surt Hypersaline spring.
Materials and Methods: In this experimental study, 42 pure bacterial colonies were isolated from different samples of water, soil, sediment, and sludge from a hypersaline spring with a screening technique on the specific culture medium of halophilic bacteria. The isolate S-14, which showed the highest lipase activity, was selected for the identification by biochemical methods and 16S rRNA gene analysis. In order to optimize the growth conditions of the isolate, considering the maximum time of bacterial growth (72 hours), temperature, salt concentration, pH, carbohydrate, and amino acid intake were examined. The results were edited by Chromas pro 2.1.1 software, and compared with EzTaxon database. Strains that were more similar to the isolate were identified. Sequence analysis of 16S rRNA were performed by BioEdit 7.1.9, Clustal-2X 2.1, and MEGA 6, and the phylogenetic tree was drawn by the neighbor joining algorithm.
Findings: The isolate S-14 had 99% similarity to Marinobacter flavimaris and Marinobacter adhaerens. The isolate had optimum growth in 5% NaCl concentration, 35°C, and 7.0 acidity.
Conclusion: The isolate S-14 can be an appropriate candidate to produce extracellular lipase enzyme and can utilize Fructose and Phenylalanine as a sole source of carbon and energy.
J. Zafari , F. Javani Jouni , M. Satari Keykaleh , P. Abdolmaleki , M.j. Khodayar , A. Jalali ,
Volume 9, Issue 4 (Fall 2018)
Abstract
Aims: Regarding the treatment of cancer, due to the limitation in the use of high dose and resistance of cancer cells, it is necessary to use optimal methods that have high therapeutic efficacy and reduce the dose of radiation and medicine. The aim of the present research was to investigate toxicity of cisplatin under the influence of static magnetic field in susceptible and drug-resistant cell.
Materials and Methods: In the present experimental study, A2780-CP resistant cell classes and susceptible to A2780 cisplatin were investigated in the field and drug-treated cell groups compared to the drug-receiving group alone, and to determine the effect of static magnetic field and concentration of drug, 10mT for 24 hours and logarithmic drug concentration (1, 10, 50, 100, and 500mcg/ml) were used. Inhibitory concentration of 50% cell growth (IC50) was obtained for the cells in the absence and presence of the magnetic field after conversion of the absorption obtained in the ELISA from the MTT test to cytotoxicity percentage. Data were analyzed with Prism software using two-way ANOVA and T-test.
Findings: In the presence of a static magnetic field and different drug concentrations, a greater reduction in the percentage of In vivo cells was observed. IC50 values for A2780 cells in the absence and presence of magnetic fields were 27.69±9.58 and 8.96±1.48μg/ml for A2780-CP, and 61.61±8.03 and 9.58±3.13μg/ml, respectively.
Conclusion: The mortality rate of the cells treated with cisplatin under the influence of the magnetic field is more in susceptible and drug-resistant cells than that of only drug use. Drug-resistance decreases in the drug-resistant cell class in the presence of a magnetic field.
F. Shariari, Sh. Moradi, M. Totonchi, L. Satarian, S.j. Mowla, H. Baharvand,
Volume 10, Issue 3 (Summer 2019)
Abstract
Aims: The retinal pigment epithelium cells (RPE) have crucial roles in the health and functionality of retina. Any damage or dysfunction of these cells can lead to severe retinopathies. Identification of signaling pathways and biological processes involved in RPE differentiation can be useful in devising more robust therapeutic approaches.
Materials and Methods: In the present study, we used the intersection of three online prediction databases and their ::union:: with one experimental database to select microRNAs gene targets. Next, by the intersect of the targeted genes with an increase in their expression in epithelial to mesenchymal transition (EMT) of RPE cells, we tried to build a microRNA-mRNA integrative network. Further, several pathway analyses tools were used to perform a more accurate and comprehensive analysis of the signaling pathways and biological processes being regulated by selected miRs in the EMT of the RPE cells.
Findings: Our study revealed that among the 3406 genes being upregulated over the course of EMT in RPE cells, adj p-value≤0.05, fold change≥1.5, 93 genes were miR-204-5p and miR 211-5p target genes. Further analysis of the obtained target gene list demonstrated that these two microRNAs are mostly involved in maintaining RPE cells from going through EMT via regulation of cell adhesion and secretion subnetworks and also MAPK and TGF-β1 signaling pathways while preserving cells from apoptosis and neuronal fates.
Conclusion: This study indicated that miR-204-5p and miR 211-5p are involved in protecting RPE cells from EMT and reinforce their epithelial cell identity.
Volume 11, Issue 0 (بهار و تابستان 87- 2008)
Abstract
Objective: Helicobacter pylori is a spiral, microaerophilic gram negative bacterium, that multiplies and causes infection in human gastric mucosal layer. H.pylori infection, followed by destruction of gastric epithelial tissue, leads to gastric chronic inflammation, which can cause gastric and peptic ulcers. New approaches have focused on using specific treatments, such as immunotherapy, to eradicate this infection. Urease, as one of the most important virulent and antigenic factors of the bacterium, is a suitable target for this purpose. This study is aimed at production of specific IgY against urease UreC subunit.
Materials and Methods: In this study, initially for preparing recombinant UreC, after purification of the genomic DNA, ureC gene was amplified by polymerase chain reaction (PCR). The PCR product was ligated to pET28a. The recombinant protein was expressed followed by transformation of recombinant construct into E. coli BL21DE3. SDS-PAGE analysis was carried out and the recombinant protein was purified by Ni-NTA affinity chromatography. The purified recombinant protein was injected to hens. IgY recovered from egg yolk, was purified by PEG precipitation at >70% purity. The purified IgY was analyzed by ELISA and SDS-PAGE.
Results: SDS-PAGE analysis revealed a good expression and >70% purification of the recombinant protein. ELISA observation demonstrated high immunogenicity of the recombinant protein.
Conclusion: With a view to higher potential of IgY-HpUc in recognition of UreC subunit, the results are in favour of the oral administration of the IgY obtained from hens immunized by H.pylori may provide a novel approach to the management of H.pylori infections.
Mohammad Satarimohammad Satari Keykeleh, Mohammad Naseh Talebi, Mehdi Fakhimi Kamran, Farzaneh Zarrin, Shadi Akbari,
Volume 14, Issue 4 (9-2023)
Abstract
With the wide spread of electromagnetic waves in living environment, concerns about the harmful effects of these waves on human health have increased. However, many studies have been conducted on the biological effects of non-ionizing radiation, but there is no certainty about the effects of these radiation, especially on the nervous system. The achieved results from studies have many differences and even conflicting results have been reported. According to the previous studies, it is not yet concluded whether non-ionizing electromagnetic radiation damaging to the nervous system. The purpose of this study is to analyze published articles about the effects of non-ionizing electromagnetic radiation on the nervous system in order to extract quantitative data on the effects of these waves. The purpose of this study is to analyze published articles about the effects of non-ionizing electromagnetic radiation on the nervous system in order to extract quantitative data on the effects of these waves. At first, the articles published in the ORSAA database were reviewed and divided into two categories: cellular and molecular parameters and neurological and cognitive parameters. The results obtained from the reviews and quantitative analyzes of the articles in the ORSAA database showed that in the category of cellular and molecular parameters, non-ionizing electromagnetic waves have the greatest effect on the change of enzyme activity and damage to proteins with 418 cases. Also, in the category of neurological and cognitive parameters, non-ionizing waves have the greatest effect on behavior and cognitive effects with 171 reported cases.
Mohammad Satari, Behnam Hajipoor, Saman Hosseinkhani, Parviz Abdolmaleki,
Volume 15, Issue 2 (5-2024)
Abstract
Gene delivery using the force of a magnetic field is called magnetofection. The purpose of this study is the synthesis and characterization of magnetic iron oxide nanoparticles (Fe3O4) as the core of the transfer agent and to investigate the effect of alternating magnetic field on transfection efficiency. For this purpose, the first magnetic nanoparticles (MNP) were synthesized by coprecipitation method. The magnetic properties of the synthesized MNP were investigated by vibrating sample magnetometer (VSM), appearance characteristics, and zeta potential of the synthesized particles were evaluated using transmission electron microscopy (TEM) and dynamic light scattering (DLS). Then, using magnetic nanoparticles (MNP), polyethylene imine (PEI) and plasmid DNA containing luciferase reporter gene (pDNA), PEI-pDNA binary complex and MNP-PEI-pDNA ternary complex were synthesized. The complexes were evaluated using DLS and gel retardation techniques. The results of DLS and gel retardation technique showed that the complexes have a suitable surface charge and polyethyleneimine is well joined to pDNA and neutralized its negative charge. Finaly, human breast cancer cell lines (MCF-7) and Hek293T cells were transfected by ternary complex in the presence of 50 Hz alternating magnetic field. Cell viability was measured using the MTT test. The obtained results showed that the transfection efficiency in the cells that were transfected with the ternary complex in the presence of alternating magnetic field increased significantly compared to the control group, without any additional toxicity (P ≤ 0.05).
Volume 17, Issue 70 (5-2020)
Abstract
Brevity (Ejaz), along with prolixity (Etanab) is one of the main topics in semantics, whichProvides the poet with important possibilities for artistic functions to accelerate the transmission of the message. Following the political and social developments of the Constitutional Era and the emergence of “Nima” and also, the change in the form and structure of the language of poetry, a group of poets considered brevity as one of the linguistic tricks, by reducing some unnecessary word processing mechanisms. The authors of this study have selected three schools of poetry, i.e.: “Nima Poetry”, “Free Verse” and “Short Poetry” to achieve the rhetorical and artistic methods and functions of Brevity (Ejaz) in contemporary poetry; and they have examined and analyzed the written works of a prominent figure from each school. For “Nima Poetry”, they examined Nima Yooshij poems; Shamloo’s works for Free Verse, and Short Poems of Mansour Ouji. Then they analyzed the methods of reaching to Brevity (Ejaz) in these 3 schools, including Deletion Brevity (i.e.: deletion of the verb; starting and ending deletion), Qasr Brevity (i.e.: Symbols and Allusion), and Grammar Brevity (Adverbs, Words Composition, and Verb Modification) that are all done with the aim of rhyming and reinforce music, avoid association with regret, emphasize a word or concept, show thought, suspend meaning, create ambiguity, create atmosphere, convey broad concepts, develop language and create strangeness and de-familiarization.
Volume 20, Issue 80 (4-2023)
Abstract
The life experience of Iranian man in the transition period shows that, despite his efforts, he is still unable to fully understand the requirements of the modern world and is constantly hesitating between the two traditional and modern worlds. The feedback of this problem is profoundly obvious in contemporary culture, so that it caused an incongruity in our history and culture. Incongruity doesn’t mean the existence of different and heterogeneous elements together, but the simultaneous presence asynchronous things. historical-cultural incongruity is evident in contemporary poetry as one of the basics of society’s culture, especially the poetry of Akhavan, one of the contemporary modern poets. This descriptive- analytical article attempts to reveal the reasons and bases of historical-cultural incongruity in contemporary poetry and its manifestation in Akhavan’s poems and exploring the historical experience of modernity in Iran in the meantime. This survey shows that Akhavan’s poem contains incongruities in three domains, including, form and content, language and format. It means that the form of poetry is modern but it’s content, instead of representing modern values, similar to European critics of modernity, criticizes west and it’s values. Furthermore, the usage of old language beside new one and concurrent use of old formats of Persian poetry and Nima’i poetry, is an indicator of incongruity in Akhavan’s poems.
