​Microalgae with stores of carbohydrates are introduced as a promising energy resource to produce In this study, a mixed culture was used for reducing the processing costs. Afterward, nitrogen starvation strategy was used to increase the storage in The application of mixed cultures enhances the economic feasibility of the process due to the elimination of culture sterilization. After harvesting and drying enzymatic hydrolysis of microalgal biomass for extraction Afterward, the enzymatic hydrolysate of microalgal biomass (25, 50, 100g/L) underwent fermentation with Saccharomyces cerevisiae and kinetic models for fermentation were studied. The inhibition of glucose substrate and product was considered in the kinetic model. AQUASIM 2.0 software was used as a tool to simulate the fermentation process. The estimated values of the maximum specific growth rate (μ) Monod constant (K_s) to be 0.281h ⁻¹ 1.8g/L, respectively. Also, the results indicate that the kinetic model predicted the behavior of the system well.