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Mutual effect of culture density and diet administration of pomegranate peel (Punica granatum L.) extracts on biochemical and antioxidant parameters of common carp (Cyprinus carpio) blood serum

Volume 11, Issue 3 (8-2022)
Abstract
Today, due to the confirmation of the existence of polyphenolic compounds with antioxidant properties in pomegranate peel, the study of its application potential in food health and fishery products has been the focus of researchers. In the present study, the effects of oral administration of ethanolic extract of pomegranate peel (Punica granatum L.) (Dose: 600 mg/kg) on functional changes of antioxidant system and biochemical parameters of blood serum of common carp (Cyprinus carpio) during a 45-day exposure against stocking density stress was monitored. In this regard, fish were randomly divided into 6 groups (3 treatment groups and 3 control groups‌ with different levels of stocking density (3, 5, and 10 kg / m3) and three replications. At the end of the exposure period, fish bloodletting and serum preparation were performed. The results of this study did not show a significant increase in biochemical parameters (phosphorus, chlorine, magnesium, calcium, and triglycerides) as well as the activity level of some enzymes and antioxidant compounds such as SOD and MDA in fish serum compared to the control group. However, for stress indicators such as glucose and cholesterol and antioxidant activity such as CAT and GSH, production level has been significant. According to the findings, oral administration of pomegranate peel extract dietary supplement is recommended for consumption in dense aquaculture centers of common carp fish.

Assessment of Low Level Laser (808 nm) Effects on E.coli -DH5α Viability and Growth
Soheila Takavar, Majid Sadeghizadeh, Heshmatollah Rahimian, Gholamreza Esmaeeli Djavid,
Volume 12, Issue 1 (12-2020)
Abstract
NIR Laser application in bacteria is often focused on mortality and antibiotic efficacy. The literature records on this point are absolutely diverse from mortality in different degrees to immortality and even viability enhancement. The aim of this study is to investigate 808 nm laser effects on E.coli-DH5α viability and Growth with CFU, MTT and FCM assays. To obtain the purpose, bacteria in LB media put on with 808nm laser on 100 and 200 J/cm2 dosages and were investigated and compared by CFU, MTT and FCM assay. CFU assay results after 24 hours incubation were not significantly different between laser treatments and control. (P=0.06). In contrast, MTT assay results after 1 hours from laser treatment indicated significant deleterious effects in 200 J/cm2 laser treatment compared with control(P=0.006). On the other hand, FCM assay results of laser treatments with using of PI and Triton X100 not only approved MTT assay results but also revealed some dose dependent changes on bacteria ranging from increase membrane permeability to lethal damages. As a conclusion of the results in these method assays, we can state that these different laser doses produce diverse effects on viability and growth in E.coli-DH5α. Consequently the laser treatments could be planned for antibiotic purposes or enhancing gene transformation process.

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