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The bioluminescence process is a widespread phenomenon in Nature. These enzymes are identified in some domains of life, but the luciferases from the lampyridea genus are considered for biological applications. The molecular cloning of a new type of Iranian firefly luciferase from Lampyroidea maculata was reported, previously. In this study, we analyzed the rare codons of the Iranian insect luciferase gene using the computational databases as ATGme, RACC, LaTcOm, and Sherlocc. Also, the structural modeling process of this enzyme was performed. Next, the status of these rare codons in this structural model was studied using SPDBV and PyMOL software. In the following, the substrate binding site was studied using the AutoDock Vina. By molecular modeling, some rare codons were identified that may have a critical role in the structure and function of this luciferase. AutoDock Vina was used in the molecular docking that recognizes Asp⁵³¹ that yield closely related to luciferin and AMP binding site.. This bioinformatics analyzes play an important role in the design of new drugs.

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This article has been attempting to present a novel and biocompatible nanocarrier based on chitosan biopolymer with high efficiency and low toxicity for gene delivery. At the first, 5-amino-1H-tetrazol reacted with (3-chloropropyl)trimethoxysilane and then, the obtained organosilane intermediate was used for functionalization of chitosan amino groups. The structure and chemical composition of the synthesized nanocarrier were fully characterized by FESEM, TEM, XRD, FTIR, Zeta potential and DLS analysis. At the fallowing, the MTT test was performed in order to investigation of the nanocarrier toxicity. Furthermore, Hek-293T cell line was used for investigation of the nanocarrier efficiency in gene transfection toward the cell. The loading capacity of plasmid was optimal at an N/P ratio of 3 with excellent protection of the plasmid. The results were shown that the novel nanocarrier is high efficient and has high potential to gene transfection and protection in addition of nontoxicity, biodegradability and cost effectively.