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Showing 8 results for Yakhchali


Volume 0, Issue 0 (in press 2024)
Abstract

Masonry infills are generally assumed as non-structural elements in structural calculations and are not modeled. However, observations after past earthquakes have shown that masonry infills have significant effects on the seismic performance of structures and their seismic behavior should not be neglected. Additionally, the absence of masonry infills in the first story, which is common in structures for commercial and architectural reasons, has led to the occurrence of the soft story phenomenon in past earthquakes. The maximum interstory drift ratio (MD) is the most important criterion for assessing seismic damage and the occurrence of collapse in structures. In this study, the seismic performance of 3- and 9-story steel moment resisting frames (MRFs) with masonry infills was evaluated using a probabilistic framework considering the record-to-record variability. Two configurations were considered for the masonry infills including fully infilled and open ground story configurations. The seismic performance of the MRFs with these two configurations was compared to that of bare MRFs. The OpenSees software was employed for nonlinear modeling of the structures and masonry infills were modeled using single compression-only struts. The fundamental periods of structures with masonry infills significantly increase after the failure of the masonry infills. To evaluate these effects, Rayleigh damping was modeled using the conventional method and a modified method, which considers the severe elongation of fundamental period due the failure of infills, and the responses obtained from the two methods were compared. By performing incremental dynamic analyses using 78 far-field ground motion records, drift margin ratios (DMRs), drift fragility curves and mean annual frequencies of exceeding four MD levels of 0.7%, 2.5%, 5%, and 15% (λMD) were obtained for the structures. The MD levels of 0.7%, 2.5%, and 5% correspond to the performance levels of immediate occupancy, life safety, and collapse prevention, respectively. The MD level of 15% corresponds to the seismic collapse of the structures. The results indicate that the presence of masonry infills improves the drift performance of the MRFs with the fully infilled configuration. However, since the masonry infills experience failure at higher drift levels, their effectiveness decreases at these drift levels. Furthermore, the absence of masonry infills in the first story leads to the soft story phenomenon at lower drift levels, and therefore, the performance of the structures with the open ground story configuration is worse than that with the fully infilled configuration. It should be mentioned that at higher drift levels, due to the failure of masonry infills, the structures with the two configurations for infills have almost the same performance and close to the performance of the bare MRFs. The amplification of the soft story phenomenon in the 9-story structure causes the performance of the structure with the open ground story configuration given some drift levels to be even worse than that of the bare structure. For example, by using the modified damping method for the 3-story structure with the fully infilled configuration, the masonry infills reduce the λMD value given MD = 0.7% by 43%, but the reduction in the λMD given MD = 15% is 19%. Based on the results obtained, the conventional Rayleigh damping method in the technical literature underestimates the responses.


Volume 2, Issue 4 (12-2013)
Abstract

Citrus tristeza virus (CTV) is among the most destructive pathogens of citrus and causes substantial economic losses in citrus-growing industry worldwide. Considering recent distribution of this pathogen and its capability of transmission by existing aphid vectors in Iran, detection of this virus is enforceable for controlling the damage caused by this pathogen in Iran, as one of the major citrus producing countries. Toward this aim, developing a reliable and sensitive detection method such as enzyme- linked immunosorbant assay (ELISA) would be the first step to detect CTV in large scale screenings of field samples. As the serological method requires great amounts of specific antibody, the consequent preparation of a large scale antigen source for immunization process is necessary. In this study the coat protein gene of CTV (CP25) was amplified by polymerase chain reaction from a cloned CP25 gene in pTZ57R/T and subcloned in pET26b expression vector and named pET-CP25. Two Escherichia coli strains of BL21 and Rosetta Gami (DE3) were transformed by pET-CP25. Expression of recombinant protein was induced by IPTG. The authenticity of recombinant protein was confirmed by western immunoblot analysis using a polyclonal antiserum against CTV particles. The results indicated that CTV coat protein gene was expressed in E.coli. This recombinant protein could be used as a source of antigen for immunization process.

Volume 4, Issue 5 (Supplementary Issue - 2015)
Abstract

Serological methods are commonly used methods for detection of viruses. Preparation of pure viral antigens is a crucial step in production of antibodies required for serological studies. In this research the gene encoding coat protein of a Beet western yellows virus (BWYV) isolate from Iran was amplified by PCR and was ligated into a bacterial expression vector (pET26b) to obtain pET-BWYV-CP clone. Escherichia coli BL21 was transformed with pET-BWYV-CP and expression of the recombinant coat protein was induced by IPTG. The expressed recombinant coat proteins were purified and used as an antigen for rabbit immunization. The antiserum was able to detect recombinant coat protein in total protein extracts of induced E. coli BL21 cells in western blot analysis.  

Volume 8, Issue 1 (6-2006)
Abstract

Purpose: In order to express human granulocyte-macrophage colony stimulating factor (hGM-CSF) under heat shock. Materials and Methods: Two expression plasmids were constructed based on pBC(SK) plasmid. The expression cassettes in the two plasmids are equipped with a 75 base pair fragment, derived from the PL promoter of the bacteriophage lambda (λ). The plasmids also contain a temperature mutant of repressor coding gene (CI857) to regulate the promoter activity. The two plasmids differ from each other in having a transcription termination signal or not, down stream to the recombinant gene in the expression cassette. A pelB signal sequence was also used in order to have the recombinant protein in the periplasmic space of Escherichia coli. The efficiency of the constructed plasmids was demonstrated by heat-regulated expression of hGM-CSF. Results and Discussion: The protein analysis of the recombinant bacteria, containing either of the two plasmids, indicates a successful expression and complete processing of the hGM-CSF precursor, following the heat shock activation of the λPL promoter. In order to enhance the applicability of the terminator containing plasmid, for the expression of other proteins of interest by heat regulation, a multiple cloning site including eleven unique restriction sites was inserted in the plasmids. The heatregulated plasmids, designed in this work, have provided suitable tools to study the expression of recombinant proteins under temperature up-shift in Escherichia coli, when the use of chemical inducers are not desirable.
R. Ghasemi , H. Hashemzadeh ‎, H. Razavi ‎, B. Yakhchali ,
Volume 9, Issue 1 (Winter 2018)
Abstract

Introduction: Growth hormone is a non-glycosylated polypeptide strand of the pituitary glands of all vertebrates that has a wide range of biological activities and considering the importance of this hormone and its importance and diverse therapeutic applications in medicine, its recombinant production can be of great importance. In recent decades, protein engineering and genetic engineering have resulted in a high level of expression and production of this protein in a variety of hosts, including Escherichia coli bacteria using new techniques and methodes, hormone purification and assay are carried out easily. Therefore, the aim of this review was to investigate the production of recombinant human growth hormone (rhGH) and future challenges.
Conclusion: One of the problems of the expression and purification of the human growth hormone may involve that maybe noted the production of inclusion bodies in the expression of recombinant proteins in the cell cytoplasm, the contamination caused by host proteins, low protein recovery from these inclusion bodies, low protein secretion into the Periplasmic space, high cost of production, especially in Purification stage and so on. Due to the lack of need for glycosylated hormone and high efficiency and simplicity of work, bacterial systems, especially Escherichia coli, are the most economical and effective systems for the expression of heterologous proteins. The hormone purification stage is usually the most costly process. Therefore, an optimal design for achieving the highest target protein recovery with the elimination of all contamination from the final product and reducing the purification step is required.
Fathollah Ahmadi, Alireza Abbasi, B. Yakhchali, Hooshang Alizade, A.a. Pourbabaee ‎,
Volume 11, Issue 1 (Winter 2020)
Abstract

Aims: Peroxidases are used in a wide range of biotechnological processes, most of which are carried out at high temperatures and high pH levels. Since most of the commonly used peroxidases are unstable and inactive in alkaline conditions and high temprature, it is necessary to find thermoalkalophilic peroxidases for practical purposes.
Materials and Methods: In this study, extracellular production of peroxidase in the native strain Bacillus tequilensis was studied. for this purpose, Enzyme activity was evaluated using two substrates 2,4-DCP and pyrogallol in bacterial liquid culture and the effect of culture time on enzyme production, as well as the effect of parameters such as pH and temperature on enzyme activity investigated. The relative purification of the enzyme was performed using ion exchange chromatography with sephadex DEAE A50 and the kinetic parameters of enzyme activity were evaluated. In this study, kinetic parameters such as Km and Vmax were calculated.
Results: Measurement of enzyme activity at different times of culture indicated that the highest amount of peroxidase production was obtained 72 h after bacterial culture.

Volume 20, Issue 6 (12-2020)
Abstract

In design of structures using force-based methods applied in current seismic codes, to obtain the nonlinear displacements of structures under the design earthquake, deflection amplification factor (Cd) is applied. In other words, the displacements obtained from elastic analyses under the reduced seismic forces are amplified by Cd to obtain the inelastic displacements under the design earthquake. Research studies showed that using a constant coefficient for estimating the inelastic displacements may lead to considerable overestimation or underestimation of the displacements in different stories of structures. Generally, in regular structures the inelastic maximum interstory drift ratio (IMIDR) occurs in lower stories. Investigating the seismic performance of structures with irregularity in their heights showed that the inelastic responses of these types of structures can differ significantly from the inelastic responses of regular structures. The present study investigates Cd for estimating IMIDR and inelastic maximum roof drift ratio (IMRDR) for steel special moment resisting frames (SMRFs) with vertical mass irregularity under the design earthquake. In addition, the variation of Cd with the variation of the location of the heavier story in the structural height, and mass ratio (i.e., the ratio of the mass of the heavier story to the mass of the adjacent story) is studied. For producing a heavier story, the dead and live loads of the story are multiplied by 2.0 and 3.0. Three different locations (i.e., bottom, mid-height and top story) for the heavier story, are assumed. For investigating the effects of mass irregularity, two regular 5- and 10-story structures are also considered. Therefore, 14 structures (i.e., two mass ratios × two building heights (5 and 10 stories) × three locations for the heavier story + two regular structures) are considered. To perform nonlinear dynamic analyses, 67 ground motion records are applied. The records are scaled such that the mean of the pseudo acceleration response spectra exceeds the design response spectrum for the period range of 0.2T1 to 1.5T1. The results show that using Cd = 5.5 recommended by Standard No. 2800 and ASCE 7 for steel SMRFs underestimates the IMIDR in most of the structures considered and their stories, under the design earthquake. When the heavier story is located in the first story, the lowest mean Cd is obtained in the first story. Because, increasing the mass of the story leads to an increment in the stiffness and strength demand of the story. When the heavier story is located at the roof, the lowest mean Cd is obtained for the top story. While the mean Cd in the first story increases significantly. Moreover, it is shown that Cd = 5.5 underestimates the IMRDR in the structures considered. Investigating the consideration of different values for Cd shows that using Cd = 7.5 leads to the lowest error in the estimation of IMIDR in the structures considered. In the case of estimating IMRDR, the displacement amplification factor is termed Cd Roof, and it is shown that using Cd Roof = 6.5 leads to the lowest error in the estimation of IMRDR. Therefore, Cd = 7.5 and Cd Roof = 6.5 are respectively proposed for more precisely estimating IMIDR and IMRDR in steel SMRFs with vertical mass irregularity.
 

Volume 24, Issue 2 (6-2024)
Abstract

Buckling restrained braced frames (BRBFs) are widely used as a lateral force resisting system due to their advantageous characteristics such as elimination of brace buckling in compression, high ductility and energy dissipation. BRBFs may have damage concentration in one or few stories during severe seismic excitations, because buckling restrained brace (BRB) yields in a certain story and the stiffness of that story significantly decreases. Drift concentration is undesirable as it can lead to general instability resulting from P-Δ effects or residual drift. For controlling damage concentration in one or few stories and achieving a uniform distribution of drifts in all stories, a new system entitled rocking buckling restrained braced frame (RBRBF) can be used. RBRBF system generates uniform story drifts over the height of structure and prevents the damage concentration in one or few stories. Unlike conventional or suspended zipper braced frames, the braces on one side of the braced span along with the adjacent columns and ties are part of a vertical truss system that is hinged at the base and designed to remain elastic until the near collapse limit state is reached. This vertical truss system works as a strong support for preventing damage concentration in one or few stories of the braced frame. The braces on the other side of the braced span are BRBs and are designed to provide energy dissipation. RBRBFs are designed according to a displacement‐based approach. The novelty of this paper is investigating the residual drift performance of this new structural system under the effect of subduction ground motion records, which have higher significant durations compared with crustal ground motion records. In this study, 4-, 8-, and 12-story structures with RBRBF and BRBF systems are considered, and their residual drift capacity values given four maximum residual interstory drift ratio (MRIDR) levels of 0.2%, 0.5%, 1.0% and 2.0% are computed using incremental dynamic analyses (IDAs). IDAs are performed on two-dimensional models of the structures using 22 pairs of short-duration crustal and long-duration subduction ground motion records. After computing the capacity values given these four MRIDR levels, the residual drift margin ratios (RDMR), and the mean annual frequencies (MAFs) of exceeding different MRIDR levels (λRD) are obtained. The results demonstrate that all the RBRBFs have better residual drift performance than the BRBFs. Based on these results, the use of RBRBF dramatically reduces BRBF weaknesses including the concentration of damage in a certain story and low post-yield stiffness. For example, the ratios of the total λRD value given MRIDR= 2.0% for the BRBF system to its corresponding value for the RBRBF system for the 4-, 8-, and 12-story structures are 21.10, 4.06, and 3.21, respectively. In addition, for most of the structures, as the MRIDR level increases, the ratio of the RDMR value under crustal records to that under subduction records increases.
 


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