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Showing 2 results for Yousefi Javan
I. Yousefi Javan , F. Gharari ,
Volume 9, Issue 4 (Fall 2018)
Abstract
Aims: Osmotic stress such as drought, salinity, and cold is one of the most important stresses. The aim of this study was to isolate and evaluate the genes of AREB and MPK2 in order to study the resistance to drought of tomato plants.
Materials and Methods: In this experimental study, seeds of two varieties of Tomato (Red Cloud) and (Peto Pride; resistant and susceptible to drought stress, respectively) were grown in drought treatment levels of -2 and -4. This study used 3 replications by a model based on a completely randomized block design. Sampling was done for Thiobarbituric acid reactive material (TBARM) for each treatment in 3 replications. Randomized and repeated sampling were done for molecular studies and the genes expression. AREB1 and MPK2 genes were studied, using bioinformatics resources and with the help of specific primers, making cDNA, PCR, and Electrophoresis. The analysis of variance test and SPSS 15 software were used
Findings: With increasing drought stress, most of morphological traits had a considerable decline, but cellular oxidative index increased with the increase of stress, so that TBARM increased. The expression of AREB1 was higher than that of MPK2 gene expression. The rate of similarity between LeAREB and kinase 2 protein sequences in resistant tomatoes was 31%.
Conclusion: With increasing drought stress, most morphological traits have a significant decline, but TBARM shows a significant increase with increasing stress. The AREB1 resistant drought gene is induced by the effects of drought stresses, while the expression of the MPK2 gene does not show a significant difference.
Volume 20, Issue 6 (11-2018)
Abstract
Saffron (Crocus sativus L.), one of the most expensive spices in the world, is used mainly as food coloring and flavoring in food industry and its effective components are also used in medicine. A collection of twenty-two cultivars of saffron grown in different regions of Iran was screened with 25 SSR and 5 SNP primers in order to determine genetic identities and genetic diversity in these cultivars. On an average, 50 alleles were amplified using SSR primers with scorable fragment sizes ranging from approximately 160 to 400 bp. Among these, 33 alleles were polymorphic thus revealing 72% of polymorphism. The genetic similarity estimated according to SSR data was scaled between 9.5 and 87.8%. In determination of genetic diversity, five polymorphic SNP markers were used. Since SNP markers are mainly bi-allelic, all SNPs showed two alleles only, suggesting the potential of SSR and SNP markers in discriminating among plants of distant genetic backgrounds. Un-weighted pair group method with arithmetic mean clustering grouped the cultivars into four groups. In this study, we tried to expand the genetic diversity of C. sativus in Iran despite their asexual reproduction. Due to the similarity of climatic conditions in Iran, a certain genetic variation was observed in saffron plants. For saffron cultivation and production of high quality crop around the world, research on genetic diversity among the large family of C. sativus adds value this product.
